Properties of Malic Enzyme from the Aerobic Methanotroph Methylosinus trichosporium.

Biocatalysis Decarboxylation Kinetics Malate Dehydrogenase / chemistry Methylosinus trichosporium / enzymology NADP / metabolism Pyruvic Acid / metabolism Recombinant Proteins / biosynthesis

Journal

Biochemistry. Biokhimiia
ISSN: 1608-3040
Titre abrégé: Biochemistry (Mosc)
Pays: United States
ID NLM: 0376536

Informations de publication

Date de publication:
Apr 2019
Historique:
entrez: 24 6 2019
pubmed: 24 6 2019
medline: 27 6 2019
Statut: ppublish

Résumé

Recombinant malic enzyme from the aerobic methanotroph Methylosinus trichosporium was obtained by heterologous expression in Escherichia coli and purified by affinity metal-chelating chromatography. The homohexameric enzyme of 6×80 kDa catalyzed the reversible reaction of oxidative decarboxylation of malate to pyruvate in the presence of mono- and divalent cations and NADP+ as a cofactor. The k

Identifiants

DOI: 10.1134/S0006297919040060 PMID: 31228930
pubmed: 31228930
pii: BCM84040532
doi: 10.1134/S0006297919040060
doi:

Substances chimiques

Recombinant Proteins 0
NADP 53-59-8
Pyruvic Acid 8558G7RUTR
Malate Dehydrogenase EC 1.1.1.37
malate dehydrogenase (decarboxylating) EC 1.1.1.39

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

390-397

Auteurs

O N Rozova (ON)

G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Moscow Region, 142290, Russia.

V N Khmelenina (VN)

G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Moscow Region, 142290, Russia. khmelenina@ibpm.pushchino.ru.

I I Mustakhimov (II)

G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Moscow Region, 142290, Russia.

S Y But (SY)

G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Moscow Region, 142290, Russia.

Yu A Trotsenko (YA)

G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Moscow Region, 142290, Russia.

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Classifications MeSH

questionsmedicales.fr › Métabolisme › Biocatalyse › Properties of Malic Enzyme from the Aerobic...
questionsmedicales.fr › Métabolisme › Décarboxylation › Properties of Malic Enzyme from the Aerobic...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Properties of Malic Enzyme from the Aerobic...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Alcohol oxidoreductases › NAD (+) and NADP (+) dependent Alcohol oxidoreductases › Malate dehydrogenase › Properties of Malic Enzyme from the Aerobic...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Methylocystaceae › Methylosinus › Methylosinus trichosporium › Properties of Malic Enzyme from the Aerobic...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › NADP › Properties of Malic Enzyme from the Aerobic...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Cétoacides › Pyruvates › Acide pyruvique › Properties of Malic Enzyme from the Aerobic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Properties of Malic Enzyme from the Aerobic...