A microfluidic device for inferring metabolic landscapes in yeast monolayer colonies.
S. cerevisiae
gene expression
metabolism
microfluidics
physics of living systems
spatial organization
yeast colony
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
01 07 2019
01 07 2019
Historique:
received:
05
05
2019
accepted:
30
06
2019
pubmed:
2
7
2019
medline:
26
2
2020
entrez:
2
7
2019
Statut:
epublish
Résumé
Microbial colonies are fascinating structures in which growth and internal organization reflect complex morphogenetic processes. Here, we generated a microfluidics device with arrays of long monolayer yeast colonies to further global understanding of how intercellular metabolic interactions affect the internal structure of colonies within defined boundary conditions. We observed the emergence of stable glucose gradients using fluorescently labeled hexose transporters and quantified the spatial correlations with intra-colony growth rates and expression of other genes regulated by glucose availability. These landscapes depended on the external glucose concentration as well as secondary gradients, for example amino acid availability. This work demonstrates the regulatory genetic networks governing cellular physiological adaptation are the key to internal structuration of cellular assemblies. This approach could be used in the future to decipher the interplay between long-range metabolic interactions, cellular development and morphogenesis in more complex systems.
Identifiants
pubmed: 31259688
doi: 10.7554/eLife.47951
pii: 47951
pmc: PMC6624017
doi:
pii:
Substances chimiques
Glucose Transport Proteins, Facilitative
0
Transcription Factors
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Commission
ID : 724813
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ICEBERG-ANR-10-BINF-06-01
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-16-CE12-0025-01
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-11-LABX-0071
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-11-IDEX-0005-01
Pays : International
Informations de copyright
© 2019, Marinkovic et al.
Déclaration de conflit d'intérêts
ZM, CV, MA, XS, JD, AL, PH No competing interests declared
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