Analysis of HLA-G expression in renal tissue in lupus nephritis: a pilot study.
Adult
Anti-Inflammatory Agents
/ administration & dosage
Biopsy
Female
Follow-Up Studies
HLA-G Antigens
/ immunology
Humans
Immunologic Factors
/ administration & dosage
Lupus Nephritis
/ drug therapy
Male
Methylprednisolone
/ administration & dosage
Middle Aged
Pilot Projects
Retrospective Studies
Rituximab
/ administration & dosage
Treatment Outcome
Young Adult
HLA-G
Systemic lupus erythematosus
immunohistochemistry
lupus nephritis
rituximab
Journal
Lupus
ISSN: 1477-0962
Titre abrégé: Lupus
Pays: England
ID NLM: 9204265
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
pubmed:
12
7
2019
medline:
16
1
2020
entrez:
12
7
2019
Statut:
ppublish
Résumé
The study aimed to investigate whether HLA-G antigen is expressed in the kidneys of patients affected by lupus nephritis (LN) and whether its detection in renal biopsies could be adopted as a marker of treatment response and prognosis. Thirty renal biopsies from patients with LN were selected and analyzed through immunohistochemistry. Laboratory and clinical data were retrospectively collected at baseline, 6 and 12 months and at the latest clinical appointment. A number of patients (63.3%) were treated with rituximab (RTX) +/- methylprednisolone in the induction phase. The expression of HLA-G in glomeruli, tubules and infiltrating cells was examined and compared between lupus patients who achieved either complete or partial renal response and those who did not respond to treatment. HLA-G staining was observed in the glomeruli of 20 of 30 samples from patients with LN. The expression of the antigen was detected in podocytes, along glomerular capillary walls, on parietal glomerular epithelial cells and within the juxtaglomerular apparatus. Seventy per cent of patients whose glomeruli expressed HLA-G achieved partial or complete response at 6 months and 75% at the latest available follow up compared with 30% and 40%, respectively, of those who did not show any expression. The pattern of staining in tubules and infiltrating cells was highly variable precluding any clinical correlation. This study demonstrates that HLA-G is expressed in renal tissue in LN. Our retrospective data suggest that its expression could correlate with response to treatment.
Sections du résumé
BACKGROUND
BACKGROUND
The study aimed to investigate whether HLA-G antigen is expressed in the kidneys of patients affected by lupus nephritis (LN) and whether its detection in renal biopsies could be adopted as a marker of treatment response and prognosis.
METHODS
METHODS
Thirty renal biopsies from patients with LN were selected and analyzed through immunohistochemistry. Laboratory and clinical data were retrospectively collected at baseline, 6 and 12 months and at the latest clinical appointment. A number of patients (63.3%) were treated with rituximab (RTX) +/- methylprednisolone in the induction phase. The expression of HLA-G in glomeruli, tubules and infiltrating cells was examined and compared between lupus patients who achieved either complete or partial renal response and those who did not respond to treatment.
RESULTS
RESULTS
HLA-G staining was observed in the glomeruli of 20 of 30 samples from patients with LN. The expression of the antigen was detected in podocytes, along glomerular capillary walls, on parietal glomerular epithelial cells and within the juxtaglomerular apparatus. Seventy per cent of patients whose glomeruli expressed HLA-G achieved partial or complete response at 6 months and 75% at the latest available follow up compared with 30% and 40%, respectively, of those who did not show any expression. The pattern of staining in tubules and infiltrating cells was highly variable precluding any clinical correlation.
CONCLUSION
CONCLUSIONS
This study demonstrates that HLA-G is expressed in renal tissue in LN. Our retrospective data suggest that its expression could correlate with response to treatment.
Identifiants
pubmed: 31291846
doi: 10.1177/0961203319860582
pmc: PMC7611013
mid: EMS127225
doi:
Substances chimiques
Anti-Inflammatory Agents
0
HLA-G Antigens
0
Immunologic Factors
0
Rituximab
4F4X42SYQ6
Methylprednisolone
X4W7ZR7023
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1091-1100Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 082291
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 098476
Pays : United Kingdom
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