Efficient RNA silencing suppression activity of Potato Mop-Top Virus 8K protein is driven by variability and positive selection.

DNA Mutational Analysis Host Microbial Interactions Immune Evasion Plant Viruses / genetics RNA Interference Selection, Genetic Viral Proteins / genetics
Natural variation Next generation sequencing Plant viruses RNA silencing Viral suppressor of RNA silencing siRNA

Journal

Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674

Informations de publication

Date de publication:
09 2019
Historique:
received: 18 04 2019
revised: 27 06 2019
accepted: 29 06 2019
pubmed: 13 7 2019
medline: 9 4 2020
entrez: 13 7 2019
Statut: ppublish

Résumé

Previously, we investigated the evolution of Potato mop-top virus (PMTV) ORFs. Results indicate that positive selection acts exclusively on an ORF encoding the 8K protein, a weak viral suppressor of RNA silencing (VSR). However, how the extraordinary variability contributes to 8K-mediated RNA silencing suppression remains unknown. Here, we characterized the RNA silencing suppression activity of the 8K protein from seven diverse isolates. We show that 8K encoded by isolate P1 exhibits stronger RNA silencing suppression activity than the 8K protein from six other isolates. Mutational analyses revealed that Ser-50 is critical for these differences. By comparing small RNA profiles we found a lower abundance of siRNAs with U residue at the 5'-terminus after expression of the P1 8K compared to expression of 8K from isolate P125, an isolate with weak VSR activity. These results provide new clues as to the role of positive selection in shaping activities of VSRs.

Identifiants

DOI: 10.1016/j.virol.2019.06.018 PMID: 31299487
pubmed: 31299487
pii: S0042-6822(19)30172-2
doi: 10.1016/j.virol.2019.06.018
pii:
doi:

Substances chimiques

Viral Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

111-121

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Pruthvi B Kalyandurg (PB)

Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Linnean Center for Plant Biology, Uppsala, Sweden.

Aminallah Tahmasebi (A)

Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Linnean Center for Plant Biology, Uppsala, Sweden; Plant Virology Research Centre, College of Agriculture, Shiraz University, Iran.

Ramesh R Vetukuri (RR)

Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden.

Sandeep K Kushwaha (SK)

Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.

Alexander A Lezzhov (AA)

Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia.

Andrey G Solovyev (AG)

Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia; Department of Virology Biological Faculty, Moscow State University, Moscow, Russia.

Laura J Grenville-Briggs (LJ)

Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden.

Eugene I Savenkov (EI)

Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Linnean Center for Plant Biology, Uppsala, Sweden. Electronic address: eugene.savenkov@slu.se.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Analyse de mutations d'ADN › Efficient RNA silencing suppression activity...
questionsmedicales.fr › Phénomènes biologiques › Interactions hôte-microbes › Efficient RNA silencing suppression activity...
questionsmedicales.fr › Phénomènes biologiques › Interactions hôte-microbes › Interactions hôte-pathogène › Échappement immunitaire › Efficient RNA silencing suppression activity...
questionsmedicales.fr › Virus › Virus des plantes › Efficient RNA silencing suppression activity...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Interférence par ARN › Efficient RNA silencing suppression activity...
questionsmedicales.fr › Phénomènes génétiques › Sélection génétique › Efficient RNA silencing suppression activity...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Efficient RNA silencing suppression activity...