Transcriptional responses of Leishmania (Leishmania) amazonensis in the presence of trivalent sodium stibogluconate.
DEG
Diffuse leishmaniasis
Hierarchical cluster analysis (HCA)
Principal components analysis (PCA)
Resistance
Transcript
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
12 Jul 2019
12 Jul 2019
Historique:
received:
15
03
2019
accepted:
06
07
2019
entrez:
14
7
2019
pubmed:
14
7
2019
medline:
20
8
2019
Statut:
epublish
Résumé
In the last decade, resistance to antimonials has become a serious problem due to the emergence of drug-resistant strains. Therefore, understanding the mechanisms used by Leishmania parasites to survive under drug pressure is essential, particularly for species of medical-veterinary importance such as L. amazonensis. Here, we used RNA-seq technology to analyse transcriptome profiles and identify global changes in gene expression between antimony-resistant and -sensitive L. amazonensis promastigotes. A total of 723 differentially expressed genes were identified between resistant and sensitive lines. Comparative transcriptomic analysis revealed that genes encoding proteins involved in metabolism (fatty acids) and stress response, as well as those associated with antimony resistance in other Leishmania species, were upregulated in the antimony-resistant line. Most importantly, we observed upregulation of genes encoding autophagy proteins, suggesting that in the presence of trivalent stibogluconate (Sb This work identified global transcriptomic changes in an in vitro-adapted strain in response to Sb
Sections du résumé
BACKGROUND
BACKGROUND
In the last decade, resistance to antimonials has become a serious problem due to the emergence of drug-resistant strains. Therefore, understanding the mechanisms used by Leishmania parasites to survive under drug pressure is essential, particularly for species of medical-veterinary importance such as L. amazonensis.
METHODS
METHODS
Here, we used RNA-seq technology to analyse transcriptome profiles and identify global changes in gene expression between antimony-resistant and -sensitive L. amazonensis promastigotes.
RESULTS
RESULTS
A total of 723 differentially expressed genes were identified between resistant and sensitive lines. Comparative transcriptomic analysis revealed that genes encoding proteins involved in metabolism (fatty acids) and stress response, as well as those associated with antimony resistance in other Leishmania species, were upregulated in the antimony-resistant line. Most importantly, we observed upregulation of genes encoding autophagy proteins, suggesting that in the presence of trivalent stibogluconate (Sb
CONCLUSIONS
CONCLUSIONS
This work identified global transcriptomic changes in an in vitro-adapted strain in response to Sb
Identifiants
pubmed: 31300064
doi: 10.1186/s13071-019-3603-8
pii: 10.1186/s13071-019-3603-8
pmc: PMC6626383
doi:
Substances chimiques
Antiprotozoal Agents
0
DNA, Protozoan
0
Antimony Sodium Gluconate
V083S0159D
Types de publication
Journal Article
Langues
eng
Pagination
348Subventions
Organisme : Departamento Administrativo de Ciencia, Tecnología e Innovación
ID : 617
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