Blastocystis subtype 1 (allele 4); Predominant subtype among tuberculosis patients in Iran.
Adult
Alleles
Blastocystis
/ classification
Blastocystis Infections
/ parasitology
DNA, Protozoan
/ genetics
Feces
/ parasitology
Female
Genetic Variation
Humans
Iran
Male
Middle Aged
Phylogeny
Prevalence
RNA, Ribosomal, 18S
/ genetics
Sequence Analysis, DNA
Socioeconomic Factors
Tuberculosis
/ parasitology
Allele discrimination
Blastocystis
Iran
Socio-economic factor
Subtyping
Tuberculosis
Journal
Comparative immunology, microbiology and infectious diseases
ISSN: 1878-1667
Titre abrégé: Comp Immunol Microbiol Infect Dis
Pays: England
ID NLM: 7808924
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
05
03
2019
revised:
08
06
2019
accepted:
10
06
2019
entrez:
14
7
2019
pubmed:
14
7
2019
medline:
30
11
2019
Statut:
ppublish
Résumé
Blastocystis and tuberculosis are two public health issues that are frequently reported in regions with low level of hygiene. Therefore, the current study aimed to investigate Blastocystis subtype and allele distribution in TB patients. Totally, 161 stool samples were taken from TB patients who were undergoing anti-MTB treatment. Stool samples were concentrated using conventional formalin-ether technique and examined using Lugol's iodine staining under light microscopy. DNA extraction was carried out and discriminative fragment was amplified and sequenced. With comparison in GenBank database, relevant subtypes and alleles were characterized and phylogenetically analyzed using MEGA v.7 and Tamura 3-parameter model. In total, from 161 stool samples, 19 samples were suspected to be Blastocystis-positive. The expected fragment was amplified in 13 (8.07%) of samples. Accordingly, 11/13 (84.62%) of Blastocystis cases settled in urban and 2/13 (15.38%) were villagers. Close-contact with animals was also seen among 7/13 (53.84%) of samples. Subtype 1 (7/13; 53.84%) was the most prevalent followed by subtype 2 (5/13; 38.46%) and subtype 3 (1/13, 7.69%). All ST1 were allele 4, while alleles 9, 11 and 12 were seen in ST2 and allele 34 was the only allele observed in ST3. All three subtypes were clearly separated, while there was no separation between sequences from TB and non-TB patients. Blastocystis ST1 was the most prevalent subtype in TB patients and there was no difference between Blastocystis isolates from TB and non-TB human subjects.
Sections du résumé
BACKGROUND
BACKGROUND
Blastocystis and tuberculosis are two public health issues that are frequently reported in regions with low level of hygiene. Therefore, the current study aimed to investigate Blastocystis subtype and allele distribution in TB patients.
METHODS
METHODS
Totally, 161 stool samples were taken from TB patients who were undergoing anti-MTB treatment. Stool samples were concentrated using conventional formalin-ether technique and examined using Lugol's iodine staining under light microscopy. DNA extraction was carried out and discriminative fragment was amplified and sequenced. With comparison in GenBank database, relevant subtypes and alleles were characterized and phylogenetically analyzed using MEGA v.7 and Tamura 3-parameter model.
RESULTS
RESULTS
In total, from 161 stool samples, 19 samples were suspected to be Blastocystis-positive. The expected fragment was amplified in 13 (8.07%) of samples. Accordingly, 11/13 (84.62%) of Blastocystis cases settled in urban and 2/13 (15.38%) were villagers. Close-contact with animals was also seen among 7/13 (53.84%) of samples. Subtype 1 (7/13; 53.84%) was the most prevalent followed by subtype 2 (5/13; 38.46%) and subtype 3 (1/13, 7.69%). All ST1 were allele 4, while alleles 9, 11 and 12 were seen in ST2 and allele 34 was the only allele observed in ST3. All three subtypes were clearly separated, while there was no separation between sequences from TB and non-TB patients.
CONCLUSION
CONCLUSIONS
Blastocystis ST1 was the most prevalent subtype in TB patients and there was no difference between Blastocystis isolates from TB and non-TB human subjects.
Identifiants
pubmed: 31300114
pii: S0147-9571(19)30111-0
doi: 10.1016/j.cimid.2019.06.005
pii:
doi:
Substances chimiques
DNA, Protozoan
0
RNA, Ribosomal, 18S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
201-206Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.