Encapsulation of hydrophobic components in dendrimersomes and decoration of their surface with proteins and nucleic acids.
Janus dendrimer vesicles
biological membrane mimic
folded protein
nucleic acid
onion-like vesicles
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
30 07 2019
30 07 2019
Historique:
pubmed:
17
7
2019
medline:
31
3
2020
entrez:
17
7
2019
Statut:
ppublish
Résumé
Reconstructing the functions of living cells using nonnatural components is one of the great challenges of natural sciences. Compartmentalization, encapsulation, and surface decoration of globular assemblies, known as vesicles, represent key early steps in the reconstitution of synthetic cells. Here we report that vesicles self-assembled from amphiphilic Janus dendrimers, called dendrimersomes, encapsulate high concentrations of hydrophobic components and do so more efficiently than commercially available stealth liposomes assembled from phospholipid components. Multilayer onion-like dendrimersomes demonstrate a particularly high capacity for loading low-molecular weight compounds and even folded proteins. Coassembly of amphiphilic Janus dendrimers with metal-chelating ligands conjugated to amphiphilic Janus dendrimers generates dendrimersomes that selectively display folded proteins on their periphery in an oriented manner. A modular strategy for tethering nucleic acids to the surface of dendrimersomes is also demonstrated. These findings augment the functional capabilities of dendrimersomes to serve as versatile biological membrane mimics.
Identifiants
pubmed: 31308223
pii: 1904868116
doi: 10.1073/pnas.1904868116
pmc: PMC6681758
doi:
Substances chimiques
Dendrimers
0
Ligands
0
Liposomes
0
Nucleic Acids
0
Proteins
0
Green Fluorescent Proteins
147336-22-9
Nitrilotriacetic Acid
KA90006V9D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
15378-15385Informations de copyright
Copyright © 2019 the Author(s). Published by PNAS.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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