Rapid Examination of Phytochrome-Phytochrome Interacting Factor (PIF) Interaction by In Vitro Coimmunoprecipitation Assay.
In vitro coimmunoprecipitation assay
Phytochrome interacting factors
Protein–protein interaction
TnT system
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
19
7
2019
pubmed:
19
7
2019
medline:
31
3
2020
Statut:
ppublish
Résumé
Phytochromes are red/far-red light receptors that perceive and transduce light signals to regulate various physiological responses. A large number of phytochrome interacting proteins have been identified using genetic and biochemical approaches. A direct interaction between phytochrome and its interacting proteins, therefore, defines one of the critical steps to initiate light signaling cascades in plants. Thus it is important to thoroughly examine the light-dependent interaction between phytochromes and putative phytochrome-interacting proteins. In this chapter, a protocol for rapid and simple light dependent in vitro coimmunoprecipitation between phytochromes and phytochrome interacting factors is described. In principle, this protocol can be adapted for other putative phytochrome interacting proteins.
Identifiants
pubmed: 31317400
doi: 10.1007/978-1-4939-9612-4_2
pmc: PMC8630752
mid: NIHMS1757734
doi:
Substances chimiques
Plant Proteins
0
Phytochrome
11121-56-5
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
21-28Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM114297
Pays : United States
Références
Plant Cell. 2014 Jun 20;26(6):2441-2456
pubmed: 24951480
Mol Cell. 2006 Aug 4;23(3):439-46
pubmed: 16885032
Mol Plant. 2017 Aug 7;10(8):1035-1046
pubmed: 28711729
Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1335-40
pubmed: 22232680
Annu Rev Plant Biol. 2008;59:281-311
pubmed: 18257712
Nature. 1999 Aug 19;400(6746):781-4
pubmed: 10466729
J Cell Biol. 1999 May 3;145(3):437-45
pubmed: 10225946
Plant Cell. 2004 Nov;16(11):3045-58
pubmed: 15486102
Plant Cell. 2015 Jan;27(1):189-201
pubmed: 25627066
Nat Commun. 2017 May 11;8:15236
pubmed: 28492231
Dev Cell. 2016 Dec 5;39(5):597-610
pubmed: 27889482
Science. 2014 Jun 6;344(6188):1160-1164
pubmed: 24904166
Plant J. 2012 Nov;72(4):537-46
pubmed: 22849408
EMBO J. 2002 May 15;21(10):2441-50
pubmed: 12006496
Plant Cell. 1999 Aug;11(8):1445-56
pubmed: 10449579
Plant J. 2005 Dec;44(6):1023-35
pubmed: 16359394
Science. 2004 Sep 24;305(5692):1937-41
pubmed: 15448264
Mol Plant. 2015 Mar;8(3):467-78
pubmed: 25744387
J Biol Chem. 2012 Jun 22;287(26):22165-72
pubmed: 22577138