Functionally significant metabolic differences between B and T lymphocyte lineages.
Adenosine Triphosphate
/ biosynthesis
Animals
B-Lymphocytes
/ cytology
Cell Differentiation
/ immunology
Cell Lineage
/ genetics
Fatty Acids
/ metabolism
Gene Expression
Glucose
/ metabolism
Glycolysis
/ genetics
Histocompatibility Antigens Class II
/ genetics
Immunophenotyping
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Organ Specificity
Oxidative Phosphorylation
Primary Cell Culture
Protein Biosynthesis
/ immunology
T-Lymphocytes
/ cytology
B-cells
RNA-seq
T-cells
metabolic differences
ribo-seq
Journal
Immunology
ISSN: 1365-2567
Titre abrégé: Immunology
Pays: England
ID NLM: 0374672
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
25
04
2019
revised:
13
06
2019
accepted:
11
07
2019
pubmed:
19
7
2019
medline:
9
1
2020
entrez:
19
7
2019
Statut:
ppublish
Résumé
Activation of B and T lymphocytes leads to major remodelling of the metabolic landscape of the cells enabling their post-activation functions. However, naive B and T lymphocytes also show metabolic differences, and the genesis, nature and functional significance of these differences are not yet well understood. Here we show that resting B-cells appeared to have lower energy demands than resting T-cells as they consumed lower levels of glucose and fatty acids and produced less ATP. Resting B-cells are more dependent on OXPHOS, while T-cells show more dependence on aerobic glycolysis. However, despite an apparently higher energy demand, T lineage cells showed lower rates of protein synthesis than equivalent B lineage stages. These metabolic differences between the two lineages were established early during lineage differentiation, and were functionally significant. Higher levels of protein synthesis in B-cells were associated with increased synthesis of MHC class II molecules and other proteins associated with antigen internalization, transport and presentation. The combination of higher energy demand and lower protein synthesis in T-cells was consistent with their higher ATP-dependent motility. Our data provide an integrated perspective of the metabolic differences and their functional implications between the B and T lymphocyte lineages.
Identifiants
pubmed: 31318442
doi: 10.1111/imm.13098
pmc: PMC6742768
doi:
Substances chimiques
Fatty Acids
0
Histocompatibility Antigens Class II
0
Adenosine Triphosphate
8L70Q75FXE
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104-120Informations de copyright
© 2019 John Wiley & Sons Ltd.
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