Stilbenoid-Mediated Epigenetic Activation of Semaphorin 3A in Breast Cancer Cells Involves Changes in Dynamic Interactions of DNA with DNMT3A and NF1C Transcription Factor.


Journal

Molecular nutrition & food research
ISSN: 1613-4133
Titre abrégé: Mol Nutr Food Res
Pays: Germany
ID NLM: 101231818

Informations de publication

Date de publication:
10 2019
Historique:
received: 18 12 2018
revised: 13 06 2019
pubmed: 22 7 2019
medline: 23 6 2020
entrez: 22 7 2019
Statut: ppublish

Résumé

Loci-specific increase in DNA methylation occurs in cancer and may underlie gene silencing. It is investigated whether dietary stilbenoids, resveratrol, and pterostilbene exert time-dependent effects on DNA methylation patterns and specifically methylation-silenced tumor suppressor genes in breast cancer cells. Following genome-wide DNA methylation analysis with Illumina-450K, changes characteristic of early and late response to stilbenoids are identified. Interestingly, often the same genes but at different CpG loci, the same gene families, or the same functional gene categories are affected. CpG loci that lose methylation in exposed cells correspond to genes functionally associated with cancer suppression. There is a group of genes, including SEMA3A, at which the magnitude of hypomethylation in response to stilbenoids rises with increasing invasive potential of cancer cells. Decreased DNA methylation at SEMA3A promoter and concomitant gene upregulation coincide with increased occupancy of active histone marks. Open chromatin upon exposure to stilbenoids may be linked to decreased DNMT3A binding followed by increased NF1C transcription factor occupancy. Sequestration of DNMT3A is possibly a result of stilbenoid-mediated increase in SALL3 expression, which was previously shown to bind and inhibit DNMT3A activity. The findings define mechanistic players in stilbenoid-mediated epigenetic reactivation of genes suppressing cancer.

Identifiants

pubmed: 31327173
doi: 10.1002/mnfr.201801386
doi:

Substances chimiques

DNMT3A protein, human 0
Homeodomain Proteins 0
NFI Transcription Factors 0
SALL3 protein, human 0
Semaphorin-3A 0
Stilbenes 0
Transcription Factors 0
transcription factor nuclear factor 1 0
pterostilbene 26R60S6A5I
DNA 9007-49-2
DNA (Cytosine-5-)-Methyltransferases EC 2.1.1.37
DNA Methyltransferase 3A EC 2.1.1.37
Resveratrol Q369O8926L

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e1801386

Informations de copyright

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Auteurs

Megan Beetch (M)

University of British Columbia, 2329 West Mall, Vancouver, BC, V6T 1Z4, Canada.

Katarzyna Lubecka (K)

Department of Biomedical Chemistry, Medical University of Lodz, al. Tadeusza Kościuszki 4, 90-419, Łódź, Poland.

Kate Shen (K)

University of British Columbia, 2329 West Mall, Vancouver, BC, V6T 1Z4, Canada.

Kirsty Flower (K)

Epigenetic Unit, Department of Surgery and Cancer, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK.

Sadaf Harandi-Zadeh (S)

University of British Columbia, 2329 West Mall, Vancouver, BC, V6T 1Z4, Canada.

Matthew Suderman (M)

School of Social and Community Medicine, MRC Integrative Epidemiology Unit, University of Bristol, Beacon House Queens Road, Bristol, ESB 1QU, UK.

James M Flanagan (JM)

Epigenetic Unit, Department of Surgery and Cancer, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK.

Barbara Stefanska (B)

University of British Columbia, 2329 West Mall, Vancouver, BC, V6T 1Z4, Canada.

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