The ABC transporter ABCG36 is required for cadmium tolerance in rice.

ATP-Binding Cassette Transporters / genetics Biological Transport Cadmium / metabolism Gene Expression Regulation, Plant Oryza / genetics Plant Proteins / genetics Plant Roots / genetics Plant Shoots / genetics
ABC transporter Cd accumulation Cd tolerance OsABCG36 admium ice

Journal

Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906

Informations de publication

Date de publication:
24 10 2019
Historique:
received: 20 01 2019
accepted: 12 07 2019
pubmed: 23 7 2019
medline: 18 8 2020
entrez: 23 7 2019
Statut: ppublish

Résumé

Cadmium (Cd) is a highly toxic heavy metal in nature, which causes severe damage to plant growth. The molecular mechanisms for Cd detoxification are poorly understood. Here, we report that a G-type ATP-binding cassette transporter, OsABCG36, is involved in Cd tolerance in rice. OsABCG36 was expressed in both roots and shoots at a low level, but expression in the roots rather than the shoots was greatly up-regulated by a short exposure to Cd. A spatial expression analysis showed that Cd-induced expression of OsABCG36 was found in both the root tip and the mature root region. Transient expression of OsABCG36 in rice protoplast cells showed that it was localized to the plasma membrane. Immunostaining showed that OsABCG36 was localized in all root cells except the epidermal cells. Knockout of OsABCG36 resulted in increased Cd accumulation in root cell sap and enhanced Cd sensitivity, but did not affect tolerance to other metals including Al, Zn, Cu, and Pb. The concentration of Cd in the shoots was similar between the knockout lines and wild-type rice. Heterologous expression of OsABCG36 in yeast showed an efflux activity for Cd, but not for Zn. Taken together, our results indicate that OsABCG36 is not involved in Cd accumulation in the shoots, but is required for Cd tolerance by exporting Cd or Cd conjugates from the root cells in rice.

Identifiants

DOI: 10.1093/jxb/erz335 PMID: 31328224 PMC: PMC6812702
pubmed: 31328224
pii: 5536686
doi: 10.1093/jxb/erz335
pmc: PMC6812702
doi:

Substances chimiques

ATP-Binding Cassette Transporters 0
Plant Proteins 0
Cadmium 00BH33GNGH

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5909-5918

Informations de copyright

© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.

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Auteurs

Shan Fu (S)

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, China.

Youshe Lu (Y)

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, China.

Xiang Zhang (X)

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, China.

Guangzhe Yang (G)

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, China.

Dong Chao (D)

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, China.

Zhigang Wang (Z)

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, China.

Mingxing Shi (M)

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, China.

Jiugeng Chen (J)

National Key Laboratory of Plant Molecular Genetics (NKLPMG), Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

Dai-Yin Chao (DY)

National Key Laboratory of Plant Molecular Genetics (NKLPMG), Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

Rongbai Li (R)

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, China.
College of Agriculture, Guangxi University, Nanning, China.

Jian Feng Ma (JF)

Institute of Plant Science and Resources, Okayama University, Chuo, Kurashiki, Japan.

Jixing Xia (J)

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, China.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Transporteurs ABC › The ABC transporter ABCG36 is required for...
questionsmedicales.fr › Métabolisme › Transport biologique › The ABC transporter ABCG36 is required for...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Cadmium › The ABC transporter ABCG36 is required for...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › The ABC transporter ABCG36 is required for...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Oryza › The ABC transporter ABCG36 is required for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › The ABC transporter ABCG36 is required for...
questionsmedicales.fr › Structures de plante › Racines de plante › The ABC transporter ABCG36 is required for...
questionsmedicales.fr › Structures de plante › Parties aériennes de plante › Pousses de plante › The ABC transporter ABCG36 is required for...