TAF-ChIP: an ultra-low input approach for genome-wide chromatin immunoprecipitation assay.
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
24
01
2019
revised:
10
07
2019
accepted:
11
07
2019
entrez:
24
7
2019
pubmed:
25
7
2019
medline:
16
1
2020
Statut:
epublish
Résumé
Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a tagmentation-assisted fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality histone profiles from low cell numbers. The data obtained from the TAF-ChIP approach are amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.
Identifiants
pubmed: 31331983
pii: 2/4/e201900318
doi: 10.26508/lsa.201900318
pmc: PMC6653780
pii:
doi:
Substances chimiques
Histones
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2019 Akhtar et al.
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