Cloning and functional characterization of a novel metallothionein gene in Antarctic sea-ice yeast (Rhodotorula mucilaginosa).
Adaptation, Physiological
/ genetics
Antarctic Regions
Antioxidants
/ isolation & purification
Base Sequence
Cloning, Molecular
Copper
/ metabolism
Escherichia coli
/ genetics
Fungal Proteins
/ genetics
Gene Expression
Ice Cover
/ microbiology
Metallothionein
/ genetics
Metals, Heavy
/ metabolism
Open Reading Frames
Recombinant Proteins
/ genetics
Rhodotorula
/ genetics
functional analysis
heavy metal
metallothionein
polar yeast
Journal
Journal of basic microbiology
ISSN: 1521-4028
Titre abrégé: J Basic Microbiol
Pays: Germany
ID NLM: 8503885
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
27
04
2019
revised:
07
06
2019
accepted:
20
06
2019
pubmed:
25
7
2019
medline:
18
12
2019
entrez:
25
7
2019
Statut:
ppublish
Résumé
Metallothionein (MT) is a low-molecular-weight protein with a high metal binding capacity and plays a key role in organism adaptation to heavy metals. In this study, a metallothionein gene was successfully cloned and sequenced from Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5. Nucleotide sequencing and analysis revealed that the gene had four exons interrupted by three introns. MTs complementary DNA (named as RmMT) had an open reading frame of 321 bp encoding a 106 amino acid protein with a predicted molecular weight of 10.3 kDa and pI of 8.49. The number of amino acids and distribution of cysteine residues indicated that RmMT was a novel family of fungal MTs. Quantitative real-time polymerase chain reaction analysis showed that RmMT expression was elevated under copper-induced stress. The RmMT gene was transferred into E. coli and the RmMT expressing bacteria showed improved tolerance to copper ion and increased accumulation of heavy metals, such as Cu
Identifiants
pubmed: 31339587
doi: 10.1002/jobm.201900240
doi:
Substances chimiques
Antioxidants
0
Fungal Proteins
0
Metals, Heavy
0
Recombinant Proteins
0
Copper
789U1901C5
Metallothionein
9038-94-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
879-889Subventions
Organisme : Science and Technology Project of Weihai
ID : WH20140209
Organisme : Key Technologies R & D Program of Shandong
ID : 2016ZDJQ0206
Organisme : Natural Scientific Research Innovation Foundation in Harbin Institute of Technology
ID : HIT.IBRSEM.2013037
Organisme : Natural Scientific Research Innovation Foundation in Harbin Institute of Technology
ID : HIT.NSRIF.2016085
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.