Analysis of Underivatized Amino Acids: Zwitterionic Hydrophilic Interaction Chromatography Combined with Triple Quadrupole Tandem Mass Spectrometry.

Amino Acids / analysis Chromatography, High Pressure Liquid / instrumentation Chromatography, Reverse-Phase / instrumentation Hydrophobic and Hydrophilic Interactions Limit of Detection Tandem Mass Spectrometry / instrumentation
Amino acid analysis HILIC MRM Qualifier transition Quantifier transition Root exudation Tandem mass spectrometry Triple quadrupole mass spectrometry Zwitterionic phase

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 27 7 2019
pubmed: 28 7 2019
medline: 9 4 2020
Statut: ppublish

Résumé

Analysis of underivatized amino acids is challenging regarding both separation and detection of this small, polar, and largely UV-inactive compounds. Additives for reversed phase chromatography such as ion pairing reagents can hamper mass spectrometric detection. Zwitterionic hydrophilic interaction chromatography using MS compatible eluents together with tandem mass spectrometry in multiple reaction monitoring mode for selective detection is an attractive approach to overcome the abovementioned issues.

Identifiants

DOI: 10.1007/978-1-4939-9639-1_29 PMID: 31347133
pubmed: 31347133
doi: 10.1007/978-1-4939-9639-1_29
doi:

Substances chimiques

Amino Acids 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

395-402

Auteurs

Madeleine Werneth (M)

Department of Chemistry, University of Natural Resources and Life Sciences-BOKU Vienna, Vienna, Austria.

Gunda Koellensperger (G)

Department of Chemistry, University of Natural Resources and Life Sciences-BOKU Vienna, Vienna, Austria.

Stephan Hann (S)

Department of Chemistry, University of Natural Resources and Life Sciences-BOKU Vienna, Vienna, Austria. stephan.hann@boku.ac.at.

Articles similaires

Motif mapping during chickpea germination reveals a complex sequential activation of different proteolytic activities.

Indrani Bera, Michael O'Sullivan, Caitriona Scaife et al.
1.00
Cicer Germination Proteolysis Seeds Plant Proteins

A sustainable and green HPLC-PDA technique for the simultaneous estimation of Post-COVID-19 syndrome co-administered drugs with greenness and whiteness assessment.

Passant M Medhat, Manal Mohamed Fouad, Hany H Monir et al.
1.00
Humans Chromatography, High Pressure Liquid Acetaminophen COVID-19 SARS-CoV-2

Aminoacid functionalised magnetite nanoparticles Fe

Spoială Angela, Motelica Ludmila, Ilie Cornelia-Ioana et al.
1.00
Magnetite Nanoparticles Tryptophan Biocompatible Materials Microbial Sensitivity Tests Humans

Uncovering the therapeutic potential of green pea waste in breast cancer: a multi-target approach utilizing LC-MS/MS metabolomics, molecular networking, and network pharmacology.

Asmaa M Khalil, Omar M Sabry, Hesham I El-Askary et al.
1.00
Humans Pisum sativum Breast Neoplasms Tandem Mass Spectrometry Plant Extracts

Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Analysis of Underivatized Amino Acids:...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Analysis of Underivatized Amino Acids:...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase inverse › Analysis of Underivatized Amino Acids:...
questionsmedicales.fr › Phénomènes chimiques › Interactions hydrophobes et hydrophiles › Analysis of Underivatized Amino Acids:...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Limite de détection › Analysis of Underivatized Amino Acids:...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Analysis of Underivatized Amino Acids:...