Purification and biochemical characterization of a new organic solvent-tolerant chitinase from Paenibacillus timonensis strain LK-DZ15 isolated from the Djurdjura Mountains in Kabylia, Algeria.

Algeria Bacterial Proteins / genetics Chitinases / genetics Chromatography, High Pressure Liquid Hydrogen-Ion Concentration Molecular Weight Paenibacillus / classification Phylogeny Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Substrate Specificity Thermodynamics
Chitinase Endo-splitting enzyme Paenibacillus timonensis

Journal

Carbohydrate research
ISSN: 1873-426X
Titre abrégé: Carbohydr Res
Pays: Netherlands
ID NLM: 0043535

Informations de publication

Date de publication:
01 Sep 2019
Historique:
received: 15 04 2019
revised: 13 07 2019
accepted: 13 07 2019
pubmed: 28 7 2019
medline: 29 1 2020
entrez: 27 7 2019
Statut: ppublish

Résumé

A new extracellular chitinase (called ChiA-Pt70) was produced and purified from a newly isolated Paenibacillus timonensis strain LK-DZ15. The maximum chitinase activity recorded after 44-h of incubation at 30 °C was 11,500 U/mL. Pure enzyme was obtained after ammonium sulphate precipitation (40-70%) followed by sequential column chromatographies on fast performance liquid chromatography (FPLC) and high performance liquid chromatography (HPLC). Based on matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis, the purified enzyme is a monomer with a molecular mass of 70,166.11 kDa. The sequence of the 25 NH

Identifiants

DOI: 10.1016/j.carres.2019.107747 PMID: 31349143
pubmed: 31349143
pii: S0008-6215(19)30237-X
doi: 10.1016/j.carres.2019.107747
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Chitinases EC 3.2.1.14

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

107747

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Merzouk Yahiaoui (M)

Department of Natural and Life Sciences (SNV), Faculty of Sciences, University of M'Sila, P.O. Box 166, M'Sila, 28000, Algeria; Laboratory of Cellular and Molecular Biology (LCBM), Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), P.O. Box 32, El Alia, Bab Ezzouar, 16111, Algeria. Electronic address: merzouk.yahiaoui@univ-msila.dz.

Hassiba Laribi-Habchi (H)

Laboratory of Functional Analysis of Chemical Processes (LFACP), Process Engineering Department, Faculty of Technology, University of Blida 1, Road of Soumaâ, P.O. Box 270, 09000, Blida, Algeria.

Khelifa Bouacem (K)

Laboratory of Cellular and Molecular Biology (LCBM), Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), P.O. Box 32, El Alia, Bab Ezzouar, 16111, Algeria; Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax, 3018, Tunisia; Department of Biochemistry and Microbiology, Faculty of Biological and Agricultural Sciences (FBAS), University Mouloud Mammeri of Tizi-Ouzou (UMMTO), P.O. Box 17, Tizi-Ouzou, 15000, Algeria.

Katia-Louiza Asmani (KL)

Department of Biochemistry and Microbiology, Faculty of Biological and Agricultural Sciences (FBAS), University Mouloud Mammeri of Tizi-Ouzou (UMMTO), P.O. Box 17, Tizi-Ouzou, 15000, Algeria.

Sondes Mechri (S)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax, 3018, Tunisia.

Mohamed Harir (M)

Department of Natural and Life Sciences (SNV), Faculty of Sciences, University of M'Sila, P.O. Box 166, M'Sila, 28000, Algeria.

Hamdi Bendif (H)

Department of Natural and Life Sciences (SNV), Faculty of Sciences, University of M'Sila, P.O. Box 166, M'Sila, 28000, Algeria.

Radia Aïssani-El Fertas (R)

Laboratory of Cellular and Molecular Biology (LCBM), Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), P.O. Box 32, El Alia, Bab Ezzouar, 16111, Algeria.

Bassem Jaouadi (B)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax, 3018, Tunisia. Electronic address: bassem.jaouadi@cbs.rnrt.tn.

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Classifications MeSH

questionsmedicales.fr › Régions géographiques › Afrique › Afrique du Nord › Algérie › Purification and biochemical characterization...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Purification and biochemical characterization...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Chitinase › Purification and biochemical characterization...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Masse moléculaire › Purification and biochemical characterization...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Paenibacillus › Purification and biochemical characterization...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Purification and biochemical characterization...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse MALDI › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes physiques › Thermodynamique › Purification and biochemical characterization...