Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation.
Algal Proteins
/ genetics
Arginine
/ pharmacology
Chlorophyceae
/ growth & development
Lipid Droplets
/ drug effects
Nitrogen
/ metabolism
PII Nitrogen Regulatory Proteins
/ genetics
Phosphotransferases (Carboxyl Group Acceptor)
/ genetics
Signal Transduction
/ drug effects
Triglycerides
/ metabolism
GLB-encoded signaling protein
Nitrogen
Nonphotosynthetic green algae
Oil droplets
Journal
Planta
ISSN: 1432-2048
Titre abrégé: Planta
Pays: Germany
ID NLM: 1250576
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
09
05
2019
accepted:
25
07
2019
pubmed:
31
7
2019
medline:
18
12
2019
entrez:
31
7
2019
Statut:
ppublish
Résumé
L-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva. Polytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate that L-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner in P. parva.
Identifiants
pubmed: 31359139
doi: 10.1007/s00425-019-03249-5
pii: 10.1007/s00425-019-03249-5
doi:
Substances chimiques
Algal Proteins
0
PII Nitrogen Regulatory Proteins
0
Triglycerides
0
Arginine
94ZLA3W45F
Phosphotransferases (Carboxyl Group Acceptor)
EC 2.7.2.-
acetylglutamate kinase
EC 2.7.2.8
Nitrogen
N762921K75
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1379-1385Subventions
Organisme : Saint-Petersburg State University Grant
ID : 1.65.38.2017
Organisme : DFG
ID : Fo195/9-2
Organisme : DFG
ID : Fo195/13-1
Organisme : Russian Science Foundation
ID : 16-14-10004-П
Références
Eur J Biochem. 2000 May;267(10):2850-8
pubmed: 10806382
Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:163-210
pubmed: 11337396
Acta Biol Med Ger. 1975;34(9):1441-6
pubmed: 1221733
Mol Microbiol. 2004 Jun;52(5):1303-14
pubmed: 15165234
Plant Cell Physiol. 2004 Dec;45(12):1768-78
pubmed: 15653795
Planta. 2005 Dec;223(1):28-39
pubmed: 16133214
New Phytol. 2009;182(1):31-48
pubmed: 19210725
Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):502-7
pubmed: 20018655
Protist. 2013 Jan;164(1):49-59
pubmed: 22578427
Mol Microbiol. 2014 Feb;91(4):751-61
pubmed: 24329683
Plant Physiol. 2014 Apr;164(4):1812-9
pubmed: 24563281
Cell. 2014 Nov 20;159(5):1188-1199
pubmed: 25416954
Mol Microbiol. 2015 Mar;95(6):1025-35
pubmed: 25557370
Protist. 2015 Nov;166(5):493-505
pubmed: 26356535
Plant Sci. 2015 Nov;240:1-9
pubmed: 26475183
Front Plant Sci. 2015 Oct 26;6:899
pubmed: 26579149
Front Microbiol. 2016 Oct 26;7:1700
pubmed: 27833596
Sci Rep. 2018 Jan 15;8(1):790
pubmed: 29335634
PLoS One. 2018 Sep 21;13(9):e0204488
pubmed: 30240454
FEBS J. 2019 Jul 9;:null
pubmed: 31287617
J Cell Biol. 1985 Mar;100(3):965-73
pubmed: 3972906
Nature. 1970 Aug 15;227(5259):680-5
pubmed: 5432063