Point-of-care detection of 16S rRNA of Staphylococcus aureus based on multiple biotin-labeled DNA probes.
Biosensing Techniques
/ methods
Biotin
/ chemistry
DNA Probes
/ chemistry
DNA, Bacterial
/ genetics
DNA, Ribosomal
/ genetics
Food Microbiology
Gold
/ chemistry
Limit of Detection
Metal Nanoparticles
Molecular Conformation
Point-of-Care Systems
RNA, Ribosomal, 16S
/ genetics
Smartphone
Staphylococcus aureus
/ genetics
Wireless Technology
16S rRNA
DNA probe
Lateral flow assay
Point-of-care detection
S. aureus
Journal
Molecular and cellular probes
ISSN: 1096-1194
Titre abrégé: Mol Cell Probes
Pays: England
ID NLM: 8709751
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
02
01
2019
revised:
10
06
2019
accepted:
25
07
2019
pubmed:
2
8
2019
medline:
7
5
2020
entrez:
2
8
2019
Statut:
ppublish
Résumé
A visual method that combines multiple biotin-labeled DNA probes and lateral-flow nucleic acid biosensor was developed to detect Staphylococcus aureus. The 16S rRNA from Staphyloccocus aureus (S. aureus), coupled with multiple biotin-labeled DNA probes, was functionalized in a signal structure for lateral-flow point-of-care detection. The secondary structure of the 16S rRNA was unwound by two specific capture probes modified by Fam and multiple bridge probes, which extended additional sequences for use as initiators. By utilizing the initiators, each target 16S rRNA with multiple DNA probes could tether a number of biotin molecules, so that a large number of streptavidin-labeled gold nanoparticles could be introduced in the lateral flow assay. The images of the lateral flow detection results obtained using a smartphone were transmitted to a computer via Wi-Fi or Bluetooth connection for quantitative processing by ImageJ. The limit of detection was 10
Identifiants
pubmed: 31369831
pii: S0890-8508(19)30001-5
doi: 10.1016/j.mcp.2019.101427
pii:
doi:
Substances chimiques
DNA Probes
0
DNA, Bacterial
0
DNA, Ribosomal
0
RNA, Ribosomal, 16S
0
Biotin
6SO6U10H04
Gold
7440-57-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101427Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.