Gene profiling and expression of major allergen Alt a 1 in Alternaria alternata and related members of the Pleosporaceae family.
Alt a 1
Alternaria
Dematiaceous fungi
Expresión génica
Gene expression
Hongos dematiáceos
Pleosporaceae
Journal
Revista iberoamericana de micologia
ISSN: 2173-9188
Titre abrégé: Rev Iberoam Micol
Pays: Spain
ID NLM: 9425531
Informations de publication
Date de publication:
Historique:
received:
16
04
2017
revised:
31
10
2017
accepted:
30
01
2018
entrez:
12
8
2019
pubmed:
12
8
2019
medline:
6
2
2020
Statut:
ppublish
Résumé
Members of the Pleosporaceae family are known as important sources of airborne allergens which are responsible for asthma and allergic diseases. The purpose of this study was to investigate the gene profiling and expression pattern of Alt a 1 in Alternaria alternata and other members of the Pleosporaceae family including Stemphylium botryosum, Ulocladium chartarum, Curvularia lunata, Cladosporium cladosporioides, and Epicoccum nigrum. Alternaria alternata and related genera were cultured on Czapek-Dox broth medium at 25°C for 21 days. The presence of Alt a 1 was assessed in fungal culture filtrates by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then confirmed by immunoblot analysis. Real-time PCR was carried out for quantitation of the Alt a 1 gene encoding corresponding protein at the transcriptional level using cDNA prepared from fungal RNA. SDS-PAGE showed protein bands ranging from 14 to 100kDa. A 14kDa band corresponding to Alt a 1 was present in A. alternata, S. botryosum and U. chartarum. The gene expression of Alt a 1 was reported in A. alternata and some other related genera. The C The presence and efficient expression of Alt a 1 gene in A. alternata and other related taxa indicate that Alt a 1 protein is a major component of the secretory machinery of Pleosporaceae family members, and it may play a crucial role in its allergenicity.
Sections du résumé
BACKGROUND
BACKGROUND
Members of the Pleosporaceae family are known as important sources of airborne allergens which are responsible for asthma and allergic diseases.
AIMS
OBJECTIVE
The purpose of this study was to investigate the gene profiling and expression pattern of Alt a 1 in Alternaria alternata and other members of the Pleosporaceae family including Stemphylium botryosum, Ulocladium chartarum, Curvularia lunata, Cladosporium cladosporioides, and Epicoccum nigrum.
METHODS
METHODS
Alternaria alternata and related genera were cultured on Czapek-Dox broth medium at 25°C for 21 days. The presence of Alt a 1 was assessed in fungal culture filtrates by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then confirmed by immunoblot analysis. Real-time PCR was carried out for quantitation of the Alt a 1 gene encoding corresponding protein at the transcriptional level using cDNA prepared from fungal RNA.
RESULTS
RESULTS
SDS-PAGE showed protein bands ranging from 14 to 100kDa. A 14kDa band corresponding to Alt a 1 was present in A. alternata, S. botryosum and U. chartarum. The gene expression of Alt a 1 was reported in A. alternata and some other related genera. The C
CONCLUSIONS
CONCLUSIONS
The presence and efficient expression of Alt a 1 gene in A. alternata and other related taxa indicate that Alt a 1 protein is a major component of the secretory machinery of Pleosporaceae family members, and it may play a crucial role in its allergenicity.
Identifiants
pubmed: 31400792
pii: S1130-1406(18)30042-1
doi: 10.1016/j.riam.2018.01.006
pii:
doi:
Substances chimiques
ALTA1 protein, Alternaria alternata
0
Allergens
0
Antigens, Fungal
0
Fungal Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
66-71Informations de copyright
Copyright © 2018 Asociación Española de Micología. Publicado por Elsevier España, S.L.U. All rights reserved.