Extracellular vesicles secreted by
Antifungal Agents
/ pharmacology
Caspofungin
/ pharmacology
Cell Survival
/ drug effects
Cell Wall
/ drug effects
Endosomal Sorting Complexes Required for Transport
/ metabolism
Extracellular Vesicles
/ drug effects
Mutation
/ genetics
Proteomics
Saccharomyces cerevisiae
/ cytology
Stress, Physiological
/ drug effects
Biotechnology
ESCRT
Fungal biology
Proteomics
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
2019
2019
Historique:
received:
10
07
2018
accepted:
02
07
2019
entrez:
21
8
2019
pubmed:
21
8
2019
medline:
21
8
2019
Statut:
epublish
Résumé
Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from
Identifiants
pubmed: 31428693
doi: 10.1038/s42003-019-0538-8
pii: 538
pmc: PMC6688994
doi:
Substances chimiques
Antifungal Agents
0
Endosomal Sorting Complexes Required for Transport
0
Caspofungin
F0XDI6ZL63
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
305Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing interests.
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