Directed Evolution Reveals the Functional Sequence Space of an Adenylation Domain Specificity Code.
Journal
ACS chemical biology
ISSN: 1554-8937
Titre abrégé: ACS Chem Biol
Pays: United States
ID NLM: 101282906
Informations de publication
Date de publication:
20 09 2019
20 09 2019
Historique:
pubmed:
21
8
2019
medline:
9
4
2020
entrez:
21
8
2019
Statut:
ppublish
Résumé
Nonribosomal peptides are important natural products biosynthesized by nonribosomal peptide synthetases (NRPSs). Adenylation (A) domains of NRPSs are highly specific for the substrate they recognize. This recognition is determined by 10 residues in the substrate-binding pocket, termed the specificity code. This finding led to the proposal that nonribosomal peptides could be altered by specificity code swapping. Unfortunately, this approach has proven, with few exceptions, to be unproductive; changing the specificity code typically results in broadened specificity or poor function. To enhance our understanding of A domain substrate selectivity, we carried out a detailed analysis of the specificity code from the A domain of EntF, an NRPS involved in enterobactin biosynthesis in
Identifiants
pubmed: 31430120
doi: 10.1021/acschembio.9b00532
pmc: PMC6800085
mid: NIHMS1053722
doi:
Substances chimiques
Ethylenediamines
0
Iron Chelating Agents
0
ethylenediamine-N,N'-bis(2-hydroxyphenylacetic acid)
1170-02-1
Serine
452VLY9402
Peptide Synthases
EC 6.3.2.-
2,3-dihydroxybenzoate - serine ligase
EC 6.3.2.14
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2044-2054Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM100346
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008349
Pays : United States
Références
Nat Commun. 2018 Sep 10;9(1):3661
pubmed: 30202038
Chem Biol. 2011 Oct 28;18(10):1290-9
pubmed: 22035798
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Biochemistry. 2006 Dec 26;45(51):15495-504
pubmed: 17176071
Chem Biol. 2006 Aug;13(8):869-79
pubmed: 16931336
J Bacteriol. 2012 Jun;194(11):2809-18
pubmed: 22447909
Biochemistry. 1998 Feb 24;37(8):2648-59
pubmed: 9485415
Nat Chem. 2018 Mar;10(3):282-287
pubmed: 29461527
Angew Chem Int Ed Engl. 2012 Jul 16;51(29):7181-4
pubmed: 22711659
Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3764-9
pubmed: 19228942
Chem Biol. 2013 Jan 24;20(1):92-101
pubmed: 23352143
J Biol Chem. 2016 Oct 21;291(43):22559-22571
pubmed: 27597544
Chem Biol. 1999 Aug;6(8):493-505
pubmed: 10421756
J Comput Chem. 2015 Feb 5;36(4):251-63
pubmed: 25448866
PLoS One. 2013 Apr 18;8(4):e62136
pubmed: 23637983
J Biol Chem. 1998 Dec 4;273(49):32857-63
pubmed: 9830033
Nature. 2016 Jan 14;529(7585):235-8
pubmed: 26762461
J Chem Phys. 2005 Jun 1;122(21):214102
pubmed: 15974723
Chem Biol. 2011 May 27;18(5):601-7
pubmed: 21609841
Proc Natl Acad Sci U S A. 2007 Jul 10;104(28):11621-6
pubmed: 17606920
J Comput Chem. 2009 Jul 30;30(10):1545-614
pubmed: 19444816
Biochemistry. 2002 Jul 30;41(30):9718-26
pubmed: 12135394
J Bacteriol. 1989 Sep;171(9):4617-22
pubmed: 2548993
Biochemistry. 2018 Jul 17;57(28):4125-4134
pubmed: 29921120
Chem Biol. 2000 Mar;7(3):211-24
pubmed: 10712928
Bioinformatics. 2017 Oct 15;33(20):3202-3210
pubmed: 28633438
PLoS One. 2013 May 17;8(5):e62946
pubmed: 23690965
Proteins. 2008 May 1;71(2):982-94
pubmed: 18004753
Genome Res. 2004 Jun;14(6):1188-90
pubmed: 15173120
EMBO J. 1997 Jul 16;16(14):4174-83
pubmed: 9250661
Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):11951-6
pubmed: 17620609
Org Biomol Chem. 2013 Jul 28;11(28):4686-94
pubmed: 23752895
Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5314-9
pubmed: 16567620
Angew Chem Int Ed Engl. 2014 Sep 15;53(38):10105-8
pubmed: 25081643
PLoS One. 2010 Mar 10;5(3):e9490
pubmed: 20224823
Biochemistry. 2010 Oct 19;49(41):8815-7
pubmed: 20845982
Cell. 2019 Mar 7;176(6):1356-1366.e10
pubmed: 30799038
Proteins. 2006 Nov 15;65(3):538-48
pubmed: 16972285
Protein Sci. 1992 Apr;1(4):549-56
pubmed: 1338974
Biochemistry. 2017 Oct 10;56(40):5380-5390
pubmed: 28880538
Proc Natl Acad Sci U S A. 1968 May;60(1):269-76
pubmed: 4297916
J Bacteriol. 1987 Sep;169(9):4154-62
pubmed: 3040679
Nucleic Acids Res. 2019 Jul 2;47(W1):W81-W87
pubmed: 31032519
Chem Biol. 2015 May 21;22(5):640-8
pubmed: 26000750