Liquid-liquid phase separation rescues the conformational stability of a DNA hairpin from pressure-stress.

DNA / chemistry Fluorescence Resonance Energy Transfer Nucleic Acid Conformation

Journal

Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838

Informations de publication

Date de publication:
16 Sep 2019
Historique:
pubmed: 23 8 2019
medline: 11 10 2019
entrez: 22 8 2019
Statut: ppublish

Résumé

We used single-molecule FRET experiments to monitor the conformational equilibrium of a DNA hairpin under pressure-stress in the presence of an aqueous two-phase system mimicking intracellular liquid-liquid phase separation (LLPS). Our data demonstrate the important role of LLPS with its immanent strong excluded volume effect in rescuing biological function under harsh environmental conditions.

Identifiants

DOI: 10.1039/c9cc04967c PMID: 31433407
pubmed: 31433407
doi: 10.1039/c9cc04967c
doi:

Substances chimiques

DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

10673-10676

Auteurs

Loana Arns (L)

Physical Chemistry I - Biophysical Chemistry, Faculty of Chemistry and Chemical Biology, TU Dortmund University, Otto-Hahn Strasse 4a, D-44227 Dortmund, Germany. roland.winter@tu-dortmund.de.
ORCID: 0000-0002-4460-7061

Roland Winter (R)

Physical Chemistry I - Biophysical Chemistry, Faculty of Chemistry and Chemical Biology, TU Dortmund University, Otto-Hahn Strasse 4a, D-44227 Dortmund, Germany. roland.winter@tu-dortmund.de.
ORCID: 0000-0002-3512-6928

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Liquid-liquid phase separation rescues the...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Transfert d'énergie par résonance de fluorescence › Liquid-liquid phase separation rescues the...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › Liquid-liquid phase separation rescues the...