Identification of phenylamide phytoalexins and characterization of inducible phenylamide metabolism in wheat.

Bipolaris sorokiniana Fusarium graminearum Phenylamide Phytoalexin Poaceae Triticum aestivum

Journal

Phytochemistry
ISSN: 1873-3700
Titre abrégé: Phytochemistry
Pays: England
ID NLM: 0151434

Informations de publication

Date de publication:
Nov 2019
Historique:
received: 07 05 2019
revised: 15 06 2019
accepted: 17 08 2019
pubmed: 27 8 2019
medline: 4 12 2019
entrez: 27 8 2019
Statut: ppublish

Résumé

Changes in specialized metabolites were analyzed in wheat leaves inoculated with Bipolaris sorokiniana, the causal agent of spot blotch of Poaceae species. HPLC analysis detected the accumulation of six compounds in B. sorokiniana-infected leaves. Of these, we purified two compounds by silica gel and ODS column chromatography and preparative HPLC, and identified them as cinnamic acid amides, N-cinnamoyl-9-hydroxy-8-oxotryptamine and N-cinnamoyl-8-oxotryptamine, by spectroscopic analyses. The remaining four compounds were predicted to be p-coumaric acid amides of hydroxyputrescine, hydroxyagmatine, hydroxydehydroagmatine, and agmatine by mass spectrometry. The accumulation of two cinnamic acid amides was also induced by Fusarium graminearum infection, and by treatment with CuCl

Identifiants

pubmed: 31450090
pii: S0031-9422(19)30414-5
doi: 10.1016/j.phytochem.2019.112098
pii:
doi:

Substances chimiques

Amides 0
Sesquiterpenes 0
Copper 789U1901C5
cupric chloride S2QG84156O
Phytoalexins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

112098

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Naoki Ube (N)

United Graduate School of Agriculture, Tottori University, Tottori 680-8553, Japan.

Daiyu Harada (D)

Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.

Yuhka Katsuyama (Y)

Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.

Kumiko Osaki-Oka (K)

Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.

Takuji Tonooka (T)

National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.

Kotomi Ueno (K)

Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.

Shin Taketa (S)

Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan.

Atsushi Ishihara (A)

Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan. Electronic address: aishihara@tottori-u.ac.jp.

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Classifications MeSH