Structural analysis of the recognition of the -35 promoter element by SigW from Bacillus subtilis.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
25
05
2019
accepted:
12
08
2019
entrez:
29
8
2019
pubmed:
29
8
2019
medline:
4
3
2020
Statut:
epublish
Résumé
Sigma factors are key proteins that mediate the recruitment of RNA polymerase to the promoter regions of genes, for the initiation of bacterial transcription. Multiple sigma factors in a bacterium selectively recognize their cognate promoter sequences, thereby inducing the expression of their own regulons. In this paper, we report the crystal structure of the σ4 domain of Bacillus subtilis SigW bound to the -35 promoter element. Purine-specific hydrogen bonds of the -35 promoter element with the recognition helix α9 of the σ4 domain occurs at three nucleotides of the consensus sequence (G-35, A-34, and G'-31 in G-35A-34A-33A-32C-31C-30T-29). The hydrogen bonds of the backbone with the α7 and α8 of the σ4 domain occurs at G'-30. These results elucidate the structural basis of the selective recognition of the promoter by SigW. In addition, comparison of SigW structures complexed with the -35 promoter element or with anti-sigma RsiW reveals that DNA recognition and anti-sigma factor binding of SigW are mutually exclusive.
Identifiants
pubmed: 31461489
doi: 10.1371/journal.pone.0221666
pii: PONE-D-19-14812
pmc: PMC6713349
doi:
Substances chimiques
Bacterial Proteins
0
DNA, Bacterial
0
SigW protein, Bacillus subtilis
0
Sigma Factor
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0221666Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Mol Microbiol. 2001 Jul;41(1):59-71
pubmed: 11454200
Mol Cell. 2002 Mar;9(3):527-39
pubmed: 11931761
Adv Microb Physiol. 2002;46:47-110
pubmed: 12073657
Mol Microbiol. 2002 Sep;45(5):1267-76
pubmed: 12207695
J Bacteriol. 2003 Nov;185(21):6358-70
pubmed: 14563871
Microbiology. 2004 Mar;150(Pt 3):591-9
pubmed: 14993308
Mol Microbiol. 2004 May;52(4):1091-105
pubmed: 15130127
Genome Res. 2004 Jun;14(6):1188-90
pubmed: 15173120
FEMS Microbiol Lett. 2004 Sep 1;238(1):227-33
pubmed: 15336426
Microbiology. 2005 May;151(Pt 5):1577-92
pubmed: 15870467
J Bacteriol. 1992 Jun;174(12):3843-9
pubmed: 1597408
PLoS Biol. 2006 Jan;4(1):e2
pubmed: 16336047
Cell. 2006 Jun 16;125(6):1069-82
pubmed: 16777598
Genes Dev. 2006 Jul 15;20(14):1911-22
pubmed: 16816000
Mol Microbiol. 2006 Sep;61(6):1569-82
pubmed: 16899079
PLoS Biol. 2006 Sep;4(9):e269
pubmed: 16903784
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3292-7
pubmed: 18287032
Mol Microbiol. 2008 May;68(3):538-46
pubmed: 18331472
Nat Protoc. 2008;3(7):1213-27
pubmed: 18600227
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Q Rev Biophys. 2009 Feb;42(1):41-81
pubmed: 19508739
Mol Microbiol. 2009 Nov;74(3):557-81
pubmed: 19737356
Mol Microbiol. 2009 Dec;74(6):1412-26
pubmed: 19889088
J Bacteriol. 2010 Feb;192(3):870-82
pubmed: 19948795
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
J Mol Biol. 2011 Oct 7;412(5):754-71
pubmed: 21371479
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):271-81
pubmed: 21460445
Annu Rev Microbiol. 2011;65:37-55
pubmed: 21639785
J Chem Inf Model. 2011 Oct 24;51(10):2778-86
pubmed: 21919503
Cell. 2011 Dec 9;147(6):1257-69
pubmed: 22136875
Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):352-67
pubmed: 22505256
Nat Struct Mol Biol. 2014 Mar;21(3):269-76
pubmed: 24531660
Annu Rev Microbiol. 2014;68:357-76
pubmed: 25002089
Biomolecules. 2015 Jun 26;5(3):1245-65
pubmed: 26131973
Genes Dev. 1989 Sep;3(9):1462-71
pubmed: 2691330
Nucleic Acids Res. 2016 Jul 8;44(W1):W351-5
pubmed: 27131377
Elife. 2017 Jan 09;6:
pubmed: 28067618
PLoS One. 2017 Mar 20;12(3):e0174284
pubmed: 28319136
Protein Sci. 2018 Jan;27(1):112-128
pubmed: 28836357
Genes Genet Syst. 2018 Apr 10;92(5):223-234
pubmed: 29343670
Nat Commun. 2019 Mar 11;10(1):1153
pubmed: 30858373
Nature. 1969 Jan 4;221(5175):43-6
pubmed: 4882047
Protein Eng. 1993 Jan;6(1):37-40
pubmed: 8433969
Nature. 1997 Nov 20;390(6657):249-56
pubmed: 9384377
J Bacteriol. 1998 Aug;180(15):3765-70
pubmed: 9683469