Efficient production of recombinant tannase in Aspergillus oryzae using an improved glucoamylase gene promoter.
AotanB gene
Aspergillus oryzae
Efficient production
Over-expression
Tannase
Journal
Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
25
06
2019
revised:
05
08
2019
accepted:
05
08
2019
pubmed:
8
9
2019
medline:
16
5
2020
entrez:
8
9
2019
Statut:
ppublish
Résumé
A tannase-encoding gene, AotanB, from Aspergillus oryzae RIB40 was overexpressed in A. oryzae AOK11 niaD-deficient mutant derived from an industrial strain under the control of an improved glucoamylase gene promoter PglaA142. The recombinant tannase, designated as rAoTanBO, was produced efficiently as an active extracellular enzyme. Purified rAoTanBO showed a smeared band with a molecular mass of approximately 80-100 kDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The rAoTanBO had a molecular mass of 65 kDa, after treatment with endo-β-N-acetylglucosaminidase H. Purified rAoTanBO exhibited maximum activity at 30-35°C and pH 6.0. The tannase activity of purified rAoTanBO towards natural and artificial substrates was 2-8 folds higher than that of the recombinant enzyme produced by Pichia pastoris, designated as rAoTanBP. N-terminus of the mature rAoTanBP had six more amino acids than the N-terminus of the mature rAoTanBO. Kinetic analyses showed that rAoTanBO had higher catalytic efficiency (k
Identifiants
pubmed: 31492608
pii: S1389-1723(19)30599-7
doi: 10.1016/j.jbiosc.2019.08.002
pii:
doi:
Substances chimiques
Recombinant Proteins
0
Carboxylic Ester Hydrolases
EC 3.1.1.-
tannase
EC 3.1.1.20
Glucan 1,4-alpha-Glucosidase
EC 3.2.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
150-154Informations de copyright
Copyright © 2019 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.