Viscosity has dichotomous effects on Bdellovibrio bacteriovorus HD100 predation.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
10
02
2019
accepted:
05
09
2019
pubmed:
10
9
2019
medline:
12
5
2020
entrez:
10
9
2019
Statut:
ppublish
Résumé
Bdellovibrio bacteriovorus HD100 is a highly motile predatory bacterium that consumes other Gram-negative bacteria for its sustenance. Here, we describe the impacts the media viscosity has both on the motility of predator and its attack rates. Experiments performed in polyethylene glycol (PEG) solutions, a linear polymer, found a viscosity of 10 mPa s (5% PEG) negatively impacted predation over a 24-h period. When the viscosity was increased to 27 mPa s (10% PEG), predation was nearly abolished. Tests with three other B. bacteriovorus strains, i.e., 109J and two natural isolates, found identical results. Short-term (2-h) experiments, however, found attack rates were improved in 1% PEG, which had a viscosity of 5.4 mPa s, using bioluminescent prey and their viabilities. In contrast, when experiments were performed in dextran, a branched polymer, no increase in predation was seen even though the viscosity was a comparable 5.1 mPa s. The enhanced attack rates in this solution coincided with a 31% increase in B. bacteriovorus HD100 swimming speeds (62 μm s
Identifiants
pubmed: 31498968
doi: 10.1111/1462-2920.14799
doi:
Substances chimiques
Culture Media
0
Dextrans
0
Polyethylene Glycols
3WJQ0SDW1A
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4675-4684Subventions
Organisme : Defense Advanced Research Projects Agency
ID : W911NF-15-2-0027
Pays : International
Organisme : National Research Foundation of Korea
ID : 2016R1D1A1A09919912
Pays : International
Organisme : National Research Foundation of Korea
ID : 2017M1A3A3A02016642
Pays : International
Informations de copyright
© 2019 Society for Applied Microbiology and John Wiley & Sons Ltd.
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