Yeast PAF1 complex counters the pol III accumulation and replication stress on the tRNA genes.
DNA Damage
/ genetics
DNA Replication
/ genetics
Gene Deletion
Histones
/ metabolism
Methylation
Nuclear Proteins
/ deficiency
RNA Polymerase III
/ metabolism
RNA, Transfer
/ genetics
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ genetics
Stress, Physiological
/ genetics
Transcription, Genetic
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 09 2019
09 09 2019
Historique:
received:
10
08
2018
accepted:
21
08
2019
entrez:
11
9
2019
pubmed:
11
9
2019
medline:
3
11
2020
Statut:
epublish
Résumé
The RNA polymerase (pol) III transcribes mostly short, house-keeping genes, which produce stable, non-coding RNAs. The tRNAs genes, highly transcribed by pol III in vivo are known replication fork barriers. One of the transcription factors, the PAF1C (RNA polymerase II associated factor 1 complex) is reported to associate with pol I and pol II and influence their transcription. We found low level PAF1C occupancy on the yeast pol III-transcribed genes, which is not correlated with nucleosome positions, pol III occupancy and transcription. PAF1C interacts with the pol III transcription complex and causes pol III loss from the genes under replication stress. Genotoxin exposure causes pol III but not Paf1 loss from the genes. In comparison, Paf1 deletion leads to increased occupancy of pol III, γ-H2A and DNA pol2 in gene-specific manner. Paf1 restricts the accumulation of pol III by influencing the pol III pause on the genes, which reduces the pol III barrier to the replication fork progression.
Identifiants
pubmed: 31501524
doi: 10.1038/s41598-019-49316-5
pii: 10.1038/s41598-019-49316-5
pmc: PMC6733944
doi:
Substances chimiques
Histones
0
Nuclear Proteins
0
PAF1 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
RNA, Transfer
9014-25-9
RNA Polymerase III
EC 2.7.7.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12892Références
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