Proline oxidation fuels mitochondrial respiration during dark-induced leaf senescence in Arabidopsis thaliana.
Arabidopsis thaliana
dark-induced leaf senescence
mitochondria
primary metabolism
proline dehydrogenase
proline metabolism
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
18 11 2019
18 11 2019
Historique:
received:
09
04
2019
accepted:
18
07
2019
pubmed:
11
9
2019
medline:
11
8
2020
entrez:
11
9
2019
Statut:
ppublish
Résumé
Leaf senescence is a form of developmentally programmed cell death that allows the remobilization of nutrients and cellular materials from leaves to sink tissues and organs. Among the catabolic reactions that occur upon senescence, little is known about the role of proline catabolism. In this study, the involvement in dark-induced senescence of proline dehydrogenases (ProDHs), which catalyse the first and rate-limiting step of proline oxidation in mitochondria, was investigated using prodh single- and double-mutants with the help of biochemical, proteomic, and metabolomic approaches. The presence of ProDH2 in mitochondria was confirmed by mass spectrometry and immunogold labelling in dark-induced leaves of Arabidopsis. The prodh1 prodh2 mutant exhibited enhanced levels of most tricarboxylic acid cycle intermediates and free amino acids, demonstrating a role of ProDH in mitochondrial metabolism. We also found evidence of the involvement and the importance of ProDH in respiration, with proline as an alternative substrate, and in remobilization of proline during senescence to generate glutamate and energy that can then be exported to sink tissues and organs.
Identifiants
pubmed: 31504781
pii: 5555536
doi: 10.1093/jxb/erz351
pmc: PMC6859731
doi:
Substances chimiques
Arabidopsis Proteins
0
Chlorophyll
1406-65-1
Proline
9DLQ4CIU6V
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6203-6214Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Références
Plant J. 2000 Oct;24(2):171-81
pubmed: 11069692
J Cell Biochem. 2009 Jul 1;107(4):759-68
pubmed: 19415679
Plant Cell. 2010 May;22(5):1549-63
pubmed: 20501910
J Exp Bot. 2016 May;67(10):3079-93
pubmed: 27122571
Plant Physiol. 2018 May;177(1):132-150
pubmed: 29523713
Methods Mol Biol. 2006;323:439-47
pubmed: 16739598
Plant Cell. 2011 Jan;23(1):381-95
pubmed: 21278122
Plant Physiol. 2011 Sep;157(1):292-304
pubmed: 21791601
Annu Rev Plant Biol. 2007;58:115-36
pubmed: 17177638
Planta. 2000 Sep;211(4):510-8
pubmed: 11030550
Plant J. 2013 Feb;73(3):429-41
pubmed: 23036115
Plant J. 2008 Jan;53(1):11-28
pubmed: 17971042
Plant Physiol. 2002 Jan;128(1):73-83
pubmed: 11788754
Biochem J. 2016 Sep 1;473(17):2623-34
pubmed: 27303048
New Phytol. 2015 Dec;208(4):1138-48
pubmed: 26180024
BMC Plant Biol. 2008 Apr 17;8:40
pubmed: 18419821
Planta. 2012 Aug;236(2):387-99
pubmed: 22391856
Front Plant Sci. 2014 Apr 29;5:163
pubmed: 24808901
Mol Plant Pathol. 2017 Oct;18(8):1164-1174
pubmed: 27526663
Plant Physiol. 2017 Sep;175(1):62-76
pubmed: 28710132
Plant Cell. 1996 Aug;8(8):1323-35
pubmed: 8776899
Plant J. 2005 May;42(4):567-85
pubmed: 15860015
Plant Cell. 2018 Feb;30(2):495-509
pubmed: 29348240
Plant Physiol. 1998 Dec;118(4):1233-41
pubmed: 9847097
J Biol Chem. 2011 Apr 1;286(13):11382-90
pubmed: 21296880
Plant Physiol. 2007 May;144(1):503-12
pubmed: 17369432
Planta. 2005 Jun;221(4):580-8
pubmed: 15654637
Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8787-91
pubmed: 8710950
Trends Plant Sci. 2010 Feb;15(2):89-97
pubmed: 20036181
Plant Physiol. 1982 Jun;69(6):1348-9
pubmed: 16662400
Front Biosci (Landmark Ed). 2012 Jan 01;17:607-20
pubmed: 22201764
BMC Plant Biol. 2010 Apr 19;10:70
pubmed: 20403182
Methods Mol Biol. 2003;236:177-88
pubmed: 14501065
Ann Bot. 2010 Jun;105(7):1159-69
pubmed: 20237111
Comp Biochem Physiol B Biochem Mol Biol. 2002 May;132(1):117-29
pubmed: 11997215
J Exp Bot. 2014 Jul;65(14):3993-4008
pubmed: 24803504
Ann Bot. 2010 Jun;105(7):1141-57
pubmed: 20299346
Front Plant Sci. 2017 Feb 27;8:233
pubmed: 28289422
Plant Cell Physiol. 2004 Mar;45(3):309-17
pubmed: 15047879
Mitochondrion. 2017 Mar;33:22-37
pubmed: 27405097
J Exp Bot. 2016 Feb;67(3):751-62
pubmed: 26547795
Tree Physiol. 1995 Nov;15(11):759-64
pubmed: 14965995
Plant Physiol. 2002 Oct;130(2):709-19
pubmed: 12376638
Plant Physiol. 2016 Dec;172(4):2132-2153
pubmed: 27744300
J Exp Bot. 2018 Feb 12;69(4):891-903
pubmed: 28992054
Planta. 2015 Feb;241(2):403-19
pubmed: 25326771
Plant Physiol. 2006 Jun;141(2):776-92
pubmed: 16603661
Nucleic Acids Res. 2012 Jan;40(Database issue):D1211-5
pubmed: 22080561
Plant Cell Environ. 2007 Dec;30(12):1523-34
pubmed: 17986154
Mol Plant. 2009 Nov;2(6):1310-24
pubmed: 19995732
Plant Cell. 2007 Nov;19(11):3418-36
pubmed: 18024567
Plant J. 2004 Aug;39(4):612-28
pubmed: 15272878
J Biol Chem. 1951 Nov;193(1):265-75
pubmed: 14907713
EMBO J. 2006 Jul 12;25(13):3133-43
pubmed: 16810321
Plant Physiol. 1949 Jan;24(1):1-15
pubmed: 16654194
Plant Cell Environ. 2016 Jun;39(6):1304-19
pubmed: 26616144
Plant Physiol. 2011 Oct;157(2):587-98
pubmed: 21841088