Programmed cell death in soybean seed coats.

Apoptosis Plant Proteins / metabolism Seeds / physiology Glycine max / enzymology
Caspase-like activity Glycine max Metacaspase-like activity Programmed cell death Seed coat Trypsin inhibitor

Journal

Plant science : an international journal of experimental plant biology
ISSN: 1873-2259
Titre abrégé: Plant Sci
Pays: Ireland
ID NLM: 9882015

Informations de publication

Date de publication:
Nov 2019
Historique:
received: 13 06 2019
revised: 15 08 2019
accepted: 22 08 2019
entrez: 16 9 2019
pubmed: 16 9 2019
medline: 27 12 2019
Statut: ppublish

Résumé

Seed coat is the tissue which establishes an interface between the seed inner tissues and external environment. Our group has shown that cowpea seed coat undergoes coordinated events of programmed cell death (PCD) during development. In relation to germinating seed coats, little is known on PCD events. The goal here was to investigate the biochemical aspects of germinating soybean seed coat, focusing on proteolytic activities related to PCD. In gel and in solution activity profiles of quiescent and germinating seed coat extracts revealed a complex pattern of caspase- and metacaspase-like cysteine protease activities. Trypsin inhibitor and reserve proteins were revealed as potential substrates for these proteases. A pancaspase inhibitor (z-VAD-CHO) affected the radicle length of seeds germinated under its presence. Ultrastructural analysis showed the absence of cell organelles in all seed coat layers after imbibition, while oligonucleosome fragments peaked at 72 h after imbibition (HAI). Altogether, the data suggest the presence of biochemical PCD hallmarks in germinating soybean seed coat and point to the involvement of the detected protease activities in processes such as reserve protein mobilization and weakening of seed coat.

Identifiants

DOI: 10.1016/j.plantsci.2019.110232 PMID: 31521224
pubmed: 31521224
pii: S0168-9452(19)30824-6
doi: 10.1016/j.plantsci.2019.110232
pii:
doi:

Substances chimiques

Plant Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

110232

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Gustavo Lemos Rocha (G)

Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociência e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes/RJ, Brazil.

Saulo Pireda (S)

Laboratório de Biologia Celular e Tecidual, Centro de Biociência e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes/RJ, Brazil.

Jucélia da Silva Araújo (J)

Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociência e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes/RJ, Brazil.

Antônia Elenir Amâncio Oliveira (AE)

Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociência e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes/RJ, Brazil.

Olga Lima Tavares Machado (O)

Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociência e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes/RJ, Brazil.

Maura da Cunha (M)

Laboratório de Biologia Celular e Tecidual, Centro de Biociência e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes/RJ, Brazil.

Clicia Grativol (C)

Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociência e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes/RJ, Brazil.

Kátia Valevski Sales Fernandes (K)

Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociência e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes/RJ, Brazil. Electronic address: cowpkat@uenf.br.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › Programmed cell death in soybean seed coats.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Programmed cell death in soybean seed coats.
questionsmedicales.fr › Aliments et boissons › Aliments › Graines › Programmed cell death in soybean seed coats.