Pre- and post-serial metagenomic analysis of gut microbiota as a prognostic factor in patients undergoing haematopoietic stem cell transplantation.
Adult
Allografts
Autografts
Bacterial Typing Techniques
Bifidobacterium
/ isolation & purification
Case-Control Studies
DNA, Bacterial
/ genetics
Dysbiosis
/ complications
Feces
/ microbiology
Female
Gastrointestinal Microbiome
Hematopoietic Stem Cell Transplantation
/ adverse effects
High-Throughput Nucleotide Sequencing
/ methods
Humans
Intestines
/ microbiology
Male
Metagenomics
/ methods
Middle Aged
Prognosis
Prospective Studies
RNA, Ribosomal, 16S
/ genetics
Survival Analysis
Transplantation Conditioning
/ methods
beta-diversity
gut microbiota
next-generation sequencing
stem cell transplantation
uniFrac analysis
Journal
British journal of haematology
ISSN: 1365-2141
Titre abrégé: Br J Haematol
Pays: England
ID NLM: 0372544
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
22
04
2019
accepted:
17
07
2019
pubmed:
1
10
2019
medline:
25
7
2020
entrez:
1
10
2019
Statut:
ppublish
Résumé
The human gut harbours diverse microorganisms, and gut dysbiosis has recently attracted attention because of its possible involvement in various diseases. In particular, the lack of diversity in the gut microbiota has been associated with complications of haematopoietic stem cell transplantation (HSCT), such as infections, acute graft-versus-host disease and relapse of primary disease, which lead to a poor prognosis. However, few studies have serially examined the composition of the intestinal microbiota after HSCT. In this study, we demonstrated, using next-generation sequencing of the bacterial 16S ribosomal RNA gene, combined with uniFrac distance analysis, that the intestinal microbiota of patients undergoing allogeneic HSCT substantially differed from that of healthy controls and recipients of autologous transplants. Faecal samples were obtained daily throughout the clinical course, before and after transplantation. Notably, the proportions of Bifidobacterium and genera categorized as butyrate-producing bacteria were significantly lower in patients with allogeneic HSCT than in healthy controls. Furthermore, among allogeneic transplant recipients, a subgroup with a preserved microbiota composition showed a benign course, whereas patients with a skewed microbiota showed a high frequency of complications and mortality after transplantation. Thus, we conclude that the stability of intestinal microbiota is critically involved in outcomes of HSCT.
Substances chimiques
DNA, Bacterial
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
438-449Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 British Society for Haematology and John Wiley & Sons Ltd.
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