Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species.
Aegilops speltoides
FISH
Hordeum vulgare
Immunostaining
Meiosis
Pollen mitosis
Secale cereale
Tissue section
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
5
10
2019
pubmed:
5
10
2019
medline:
15
12
2020
Statut:
ppublish
Résumé
In this chapter we describe protocols for immunolabeling and FISH of pollen grains undergoing postmeiotic mitosis using Aegilops speltoides, Secale cereale, and Hordeum vulgare as models. Tissue sectioning of pollen overcomes the problem of the pollen grain wall impermeability that interferes with immunolocalization and in situ hybridization. The crucial element of the protocol is the generation and immobilization of tissue sections. Our method facilitates the investigation of the microspore cell divisions and pollen grain maturation.
Identifiants
pubmed: 31583671
doi: 10.1007/978-1-4939-9818-0_24
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM