Phosphoregulation of tropomyosin is crucial for actin cable turnover and division site placement.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
04 11 2019
04 11 2019
Historique:
received:
17
09
2018
revised:
29
11
2018
accepted:
30
08
2019
pubmed:
11
10
2019
medline:
14
5
2020
entrez:
11
10
2019
Statut:
ppublish
Résumé
Tropomyosin is a coiled-coil actin binding protein key to the stability of actin filaments. In muscle cells, tropomyosin is subject to calcium regulation, but its regulation in nonmuscle cells is not understood. Here, we provide evidence that the fission yeast tropomyosin, Cdc8, is regulated by phosphorylation of a serine residue. Failure of phosphorylation leads to an increased number and stability of actin cables and causes misplacement of the division site in certain genetic backgrounds. Phosphorylation of Cdc8 weakens its interaction with actin filaments. Furthermore, we show through in vitro reconstitution that phosphorylation-mediated release of Cdc8 from actin filaments facilitates access of the actin-severing protein Adf1 and subsequent filament disassembly. These studies establish that phosphorylation may be a key mode of regulation of nonmuscle tropomyosins, which in fission yeast controls actin filament stability and division site placement.
Identifiants
pubmed: 31597679
pii: jcb.201809089
doi: 10.1083/jcb.201809089
pmc: PMC6829654
doi:
Substances chimiques
Actins
0
Tropomyosin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3548-3559Subventions
Organisme : Medical Research Council
ID : MR/K001000/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203276/Z/16/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT101885MA
Pays : United Kingdom
Informations de copyright
© 2019 Palani et al.
Références
Cell Rep. 2018 Jul 10;24(2):503-514
pubmed: 29996109
Nature. 1975 Jan 10;253(5487):126-7
pubmed: 1110758
J Biol Chem. 1971 Aug 10;246(15):4866-71
pubmed: 4254541
Cell. 1989 Apr 21;57(2):233-42
pubmed: 2649250
Methods Enzymol. 1991;194:795-823
pubmed: 2005825
Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20820-5
pubmed: 23213249
Nat Commun. 2015 Nov 13;6:8707
pubmed: 26566078
Curr Biol. 2010 Aug 24;20(16):1415-22
pubmed: 20705466
J Biol Chem. 1981 Jan 25;256(2):575-8
pubmed: 6450206
Genes Dev. 1996 Nov 1;10(21):2707-19
pubmed: 8946912
Circ Res. 1974 Sep;35 Suppl 3:74-82
pubmed: 4278275
PLoS One. 2010 Dec 23;5(12):e15801
pubmed: 21203426
J Muscle Res Cell Motil. 2001;22(1):5-49
pubmed: 11563548
Adv Protein Chem. 2005;71:121-59
pubmed: 16230111
J Cell Sci. 2006 Nov 15;119(Pt 22):4710-8
pubmed: 17077120
Elife. 2017 Mar 10;6:
pubmed: 28282023
Curr Biol. 2016 Aug 8;26(15):R719-R737
pubmed: 27505246
FEBS Lett. 1987 Mar 23;213(2):316-8
pubmed: 3556584
Genes Dev. 1998 May 1;12(9):1356-70
pubmed: 9573052
Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10150-5
pubmed: 21642532
Nat Protoc. 2007;2(8):1896-906
pubmed: 17703201
Int Rev Cell Mol Biol. 2015;318:255-91
pubmed: 26315888
Nat Rev Mol Cell Biol. 2010 Feb;11(2):149-55
pubmed: 20094054
Mol Cell Proteomics. 2015 May;14(5):1275-87
pubmed: 25720772
Nature. 1980 Dec 4;288(5790):455-9
pubmed: 6893736
J Cell Sci. 2015 Aug 15;128(16):2965-74
pubmed: 26240174
Nat Protoc. 2016 Dec;11(12):2301-2319
pubmed: 27809316
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8496-501
pubmed: 11438684
Curr Biol. 2006 Dec 19;16(24):2480-7
pubmed: 17140794
Proc Jpn Acad Ser B Phys Biol Sci. 2015;91(7):321-50
pubmed: 26194856
Elife. 2013 Jan 08;2:e00116
pubmed: 23326639
Curr Biol. 2018 Mar 19;28(6):955-962.e3
pubmed: 29502950
Dev Cell. 2003 Nov;5(5):723-34
pubmed: 14602073
Dev Cell. 2007 Jun;12(6):987-96
pubmed: 17543869
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
Curr Biol. 2014 Feb 17;24(4):428-33
pubmed: 24508166
J Cell Sci. 1996 Jan;109 ( Pt 1):131-42
pubmed: 8834798
J Biol Chem. 1995 Feb 24;270(8):3463-6
pubmed: 7876075
Biophys J. 1975 Jul;15(7):707-23
pubmed: 806311
Methods Cell Biol. 1982;24:271-89
pubmed: 7098993
Cell. 1995 Aug 11;82(3):435-44
pubmed: 7634333
Int Rev Cell Mol Biol. 2008;267:183-206
pubmed: 18544499
Nature. 1992 Nov 5;360(6399):84-7
pubmed: 1436080