Spatiotemporal immune zonation of the human kidney.
Adult
Animals
Epithelial Cells
/ cytology
Female
Fetus
Gene Expression Regulation, Developmental
Humans
Kidney
/ anatomy & histology
Lymphocytes
/ cytology
Macrophages
/ cytology
Mice, Inbred C57BL
Mice, Transgenic
Myeloid Cells
/ cytology
Neutrophils
/ cytology
RNA-Seq
Single-Cell Analysis
Urinary Tract Infections
/ immunology
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
27 09 2019
27 09 2019
Historique:
received:
05
03
2018
revised:
31
01
2019
accepted:
04
09
2019
entrez:
12
10
2019
pubmed:
12
10
2019
medline:
31
3
2020
Statut:
ppublish
Résumé
Tissue-resident immune cells are important for organ homeostasis and defense. The epithelium may contribute to these functions directly or by cross-talk with immune cells. We used single-cell RNA sequencing to resolve the spatiotemporal immune topology of the human kidney. We reveal anatomically defined expression patterns of immune genes within the epithelial compartment, with antimicrobial peptide transcripts evident in pelvic epithelium in the mature, but not fetal, kidney. A network of tissue-resident myeloid and lymphoid immune cells was evident in both fetal and mature kidney, with postnatal acquisition of transcriptional programs that promote infection-defense capabilities. Epithelial-immune cross-talk orchestrated localization of antibacterial macrophages and neutrophils to the regions of the kidney most susceptible to infection. Overall, our study provides a global overview of how the immune landscape of the human kidney is zonated to counter the dominant immunological challenge.
Identifiants
pubmed: 31604275
pii: 365/6460/1461
doi: 10.1126/science.aat5031
pmc: PMC7343525
mid: EMS86701
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1461-1466Subventions
Organisme : Cancer Research UK
ID : 27176
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N024907/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S035842/1
Pays : United Kingdom
Organisme : Department of Health
ID : RP-2017-08-ST2-002
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Type : CommentIn
Informations de copyright
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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