Malpighian tubules of caterpillars: blending RNAseq and physiology to reveal regional functional diversity and novel epithelial ion transport control mechanisms.


Journal

The Journal of experimental biology
ISSN: 1477-9145
Titre abrégé: J Exp Biol
Pays: England
ID NLM: 0243705

Informations de publication

Date de publication:
21 11 2019
Historique:
received: 01 08 2019
accepted: 16 10 2019
pubmed: 23 10 2019
medline: 18 8 2020
entrez: 23 10 2019
Statut: epublish

Résumé

The Malpighian tubules (MTs) and hindgut constitute the functional kidney of insects. MTs are outpouchings of the gut and in most insects demonstrate proximodistal heterogeneity in function. In most insects, such heterogeneity is confined to ion/fluid secretion in the distal portion and ion/fluid reabsorption in the proximal portion. In contrast, MTs of larval Lepidoptera (caterpillars of butterflies and moths) are composed of five regions that differ in their association with the gut, their structure and ion/fluid transport function. Recent studies have shown that several regions can rapidly and reversibly switch between ion secretion and reabsorption. The present study employed RNAseq, pharmacology and electrophysiology to characterize four distinct regions of the MT in larval

Identifiants

pubmed: 31636157
pii: jeb.211623
doi: 10.1242/jeb.211623
pii:
doi:

Substances chimiques

Sodium 9NEZ333N27
Potassium RWP5GA015D

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2019. Published by The Company of Biologists Ltd.

Déclaration de conflit d'intérêts

Competing interestsThe authors declare no competing or financial interests.

Auteurs

Dennis Kolosov (D)

Department of Biology, McMaster University, 524 Life Sciences Building, 1280 Main St West, Hamilton, Ontario, Canada L8S4K1 kolosovd@mcmaster.ca.

Michael J O'Donnell (MJ)

Department of Biology, McMaster University, 524 Life Sciences Building, 1280 Main St West, Hamilton, Ontario, Canada L8S4K1.

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Classifications MeSH