R-SNARE FgSec22 is essential for growth, pathogenicity and DON production of Fusarium graminearum.
Cell wall integrity
Fusarium head blight
Membrane fusion
Phenotypic characterization
Vesicle trafficking
Journal
Current genetics
ISSN: 1432-0983
Titre abrégé: Curr Genet
Pays: United States
ID NLM: 8004904
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
22
08
2019
accepted:
09
10
2019
revised:
02
10
2019
pubmed:
2
11
2019
medline:
2
1
2021
entrez:
1
11
2019
Statut:
ppublish
Résumé
SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) facilitate intracellular vesicle trafficking and membrane fusion in eukaryotic cells, and play a vital role in growth, development and pathogenicity of phytopathogens. Fusarium head blight (FHB) caused by F. graminearum is one of the most devastating diseases of wheat and barley worldwide. Sec22 is a member of the SNARE family of proteins and its homologues have been shown to have diverse biological roles in different organisms. However, the functions of this protein in the development and pathogenesis of F. graminearum are currently unknown. In this study, we employed integrated biochemical, microbiological and molecular genetic approaches to investigate the roles of FgSec22 in F. graminearum. Our data reveal that this SNARE protein is localized to endoplasmic reticulum (ER) and is indispensable for normal conidiation, conidial morphology and pathogenesis of this phytopathogenic fungus. Our biochemical assay of deoxynivalenol (DON) reveals the active involvement of this protein in the production of this mycotoxin in F. graminearum. This has further been confirmed by qRT-PCR analyses of trichothecene (TRI) genes' expression where the ΔFgsec22 deletion mutant demonstrated a significant down-regulation of these genes in comparison to the wild-type PH-1. Unlike the wild-type and the complemented strain, the mutant strain presents a remarkable defect in colony formation which reflects the critical role it plays in vegetative growth. Collectively, our data support that the SNARE protein FgSec22 is required for vegetative growth, pathogenesis and DON biosynthesis in F. graminearum.
Identifiants
pubmed: 31667538
doi: 10.1007/s00294-019-01037-y
pii: 10.1007/s00294-019-01037-y
doi:
Substances chimiques
Fungal Proteins
0
R-SNARE Proteins
0
Trichothecenes
0
deoxynivalenol
JT37HYP23V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
421-435Références
Interdiscip Toxicol. 2010 Sep;3(3):94-9
pubmed: 21217881
Nat Commun. 2016 Sep 13;7:12799
pubmed: 27619642
Fungal Biol. 2015 Dec;119(12):1158-1169
pubmed: 26615739
Int J Food Microbiol. 2007 Oct 20;119(1-2):126-30
pubmed: 17716767
Plant J. 2011 Apr;66(2):268-79
pubmed: 21205036
PLoS One. 2011 Jan 24;6(1):e16439
pubmed: 21283626
Mol Plant Microbe Interact. 2014 Jun;27(6):557-66
pubmed: 24450772
J Biol Chem. 2015 Mar 20;290(12):7943-51
pubmed: 25670863
Autophagy. 2011 Dec;7(12):1570-2
pubmed: 22024744
AMB Express. 2018 Mar 12;8(1):37
pubmed: 29532188
Sci Rep. 2015 Feb 23;5:8504
pubmed: 25703795
Mol Plant Pathol. 2012 May;13(4):399-413
pubmed: 22098555
Curr Opin Cell Biol. 2013 Aug;25(4):428-33
pubmed: 23478217
EMBO J. 2000 May 2;19(9):1974-86
pubmed: 10790364
Appl Environ Microbiol. 2004 Apr;70(4):2044-51
pubmed: 15066795
Cold Spring Harb Perspect Biol. 2013 Jun 01;5(6):
pubmed: 23732476
Arch Microbiol. 2017 Aug;199(6):945-952
pubmed: 28357472
Nanomedicine. 2017 Oct;13(7):2263-2266
pubmed: 28673853
Res Microbiol. 2013 May;164(4):300-9
pubmed: 23376292
Eukaryot Cell. 2009 Jun;8(6):867-76
pubmed: 19377037
Fungal Genet Biol. 2010 Apr;47(4):364-72
pubmed: 20102747
Food Chem. 2016 Apr 1;196:445-50
pubmed: 26593513
Lett Appl Microbiol. 2014 Jul;59(1):99-107
pubmed: 24635164
Fungal Genet Biol. 2009 Aug;46(8):604-13
pubmed: 19406250
Mol Plant Microbe Interact. 2002 Nov;15(11):1119-27
pubmed: 12423017
RNA. 2017 Mar;23(3):297-307
pubmed: 27974622
Proc Natl Acad Sci U S A. 2018 Jun 12;115(24):6309-6314
pubmed: 29844177
Mol Plant Microbe Interact. 2010 Apr;23(4):522-33
pubmed: 20192838
Sci Rep. 2017 Mar 13;7:44296
pubmed: 28287158
Environ Microbiol. 2016 Nov;18(11):3689-3701
pubmed: 26940955
Environ Microbiol. 2015 Apr;17(4):1377-96
pubmed: 25186614
Mol Plant Microbe Interact. 2012 Nov;25(11):1408-18
pubmed: 22835271
Nat Commun. 2013;4:2092
pubmed: 23817436
Mol Plant Pathol. 2016 Jan;17(1):108-19
pubmed: 25880818
J Biol Chem. 2015 Apr 24;290(17):10657-66
pubmed: 25750128
Int J Mol Sci. 2017 Dec 24;19(1):
pubmed: 29295552
Curr Genet. 2018 Feb;64(1):285-301
pubmed: 28918485
Mol Gen Genet. 1999 Jul;261(6):977-84
pubmed: 10485289
Front Plant Sci. 2012 Jul 02;3:143
pubmed: 22876251
Plant Physiol. 2005 Nov;139(3):1244-54
pubmed: 16244155
Plant Cell. 2014 May 21;26(5):2265-2281
pubmed: 24850852
Curr Genet. 2005 Jan;47(1):29-36
pubmed: 15549317
Mol Plant Pathol. 2004 Nov 1;5(6):515-25
pubmed: 20565626
Toxins (Basel). 2013 Dec 19;6(1):1-19
pubmed: 24451843
J Agric Food Chem. 2014 Jun 4;62(22):4969-78
pubmed: 24820850
Nat Rev Mol Cell Biol. 2016 Aug;17(8):465-79
pubmed: 27301672
Curr Genet. 2019 Feb;65(1):153-166
pubmed: 29947970
PLoS Pathog. 2012;8(5):e1002724
pubmed: 22693448
Biochem Biophys Res Commun. 2015 Aug 7;463(4):483-9
pubmed: 26002470
Mol Plant Microbe Interact. 2018 Jun;31(6):651-664
pubmed: 29419372
PLoS One. 2010 Oct 06;5(10):e13193
pubmed: 20949084
Mol Plant Pathol. 2018 Mar;19(3):552-563
pubmed: 28142217
Front Physiol. 2017 Jan 20;8:5
pubmed: 28163686
PLoS One. 2012;7(11):e49495
pubmed: 23166686
Plant Biotechnol J. 2018 May;16(5):1024-1033
pubmed: 28973784
Fungal Genet Biol. 2004 Nov;41(11):973-81
pubmed: 15465386
Nat Rev Mol Cell Biol. 2013 Jun;14(6):382-92
pubmed: 23698585
Lett Appl Microbiol. 2014 Oct;59(4):377-83
pubmed: 24863673
G3 (Bethesda). 2015 Apr 14;5(6):1233-45
pubmed: 25873638
Phytopathology. 2008 Sep;98(9):999-1011
pubmed: 18943738
Nat Commun. 2014 Apr 14;5:3653
pubmed: 24728174
PLoS Pathog. 2009 Apr;5(4):e1000401
pubmed: 19390617
Sci Rep. 2016 Mar 01;6:22439
pubmed: 26928570
Biochem Biophys Res Commun. 2007 Feb 9;353(2):412-7
pubmed: 17188234