Comparison of citrus pectin and apple pectin in conjugation with soy protein isolate (SPI) under controlled dry-heating conditions.
Conjugate
Emulsifying property
Maillard reaction
Pectin
Solubility
Soy protein isolate
Structure
Journal
Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639
Informations de publication
Date de publication:
30 Mar 2020
30 Mar 2020
Historique:
received:
08
07
2019
revised:
04
09
2019
accepted:
07
09
2019
pubmed:
5
11
2019
medline:
15
2
2020
entrez:
3
11
2019
Statut:
ppublish
Résumé
In this study, we selected two most commonly-available commercial pectin, i.e. citrus pectin and apple pectin as the grafting polysaccharides to prepare soy protein isolate-pectin conjugates. Despite the similar degrees of methoxylation and acetylation for two pectin samples, apple pectin showed much more complex structures compared to citrus pectin, with a 2.20-fold higher molecular weight and large numbers of side chains. The conjugates were prepared under controlled dry-heating conditions and achieved the degree of graft of 25.00% and 21.85% for citrus and apple pectin, respectively. Formation of the conjugates was further confirmed by SDS-PAGE gel electrophoresis and IR spectra. Attributed to the strong steric-hindrance effect of pectin, the fluorescence intensity and surface hydrophobicity of the soy protein isolate were significantly decreased after Maillard reaction. However, both solubility and emulsifying properties of the conjugates were significantly improved. Results indicated that both pectin samples played favorable roles in protein modification.
Identifiants
pubmed: 31677451
pii: S0308-8146(19)31619-X
doi: 10.1016/j.foodchem.2019.125501
pii:
doi:
Substances chimiques
Emulsions
0
Soybean Proteins
0
citrus pectin
47EQO8LE7H
Pectins
89NA02M4RX
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
125501Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.