HepG2-1A2 C2 and C7: Lentivirus vector-mediated stable and functional overexpression of cytochrome P450 1A2 in human hepatoblastoma cells.
Aflatoxin B1
/ toxicity
Cell Line, Tumor
Cytochrome P-450 CYP1A2
/ biosynthesis
DNA Fingerprinting
/ methods
Genetic Vectors
/ genetics
Hepatoblastoma
/ enzymology
Humans
Lentivirus
/ genetics
Liver
/ metabolism
Liver Neoplasms
/ enzymology
Mycoplasma
/ chemistry
Phenacetin
/ pharmacokinetics
Plasmids
/ genetics
CYP1A2
Cytochrome P450
HepG2
Liver metabolism
Journal
Toxicology letters
ISSN: 1879-3169
Titre abrégé: Toxicol Lett
Pays: Netherlands
ID NLM: 7709027
Informations de publication
Date de publication:
01 Feb 2020
01 Feb 2020
Historique:
received:
13
08
2019
revised:
09
10
2019
accepted:
05
11
2019
pubmed:
11
11
2019
medline:
31
12
2019
entrez:
10
11
2019
Statut:
ppublish
Résumé
Novel HepG2 cell clones 1A2 C2 and 1A2 C7 were independently generated by lentiviral transduction to functionally overexpress cytochrome P450 1A2 (CYP1A2). We found similar and stable CYP1A2 transcript and protein levels in both cell clones leading to specific enzyme activities of about 370 pmol paracetamol x min
Identifiants
pubmed: 31706005
pii: S0378-4274(19)30358-3
doi: 10.1016/j.toxlet.2019.11.006
pii:
doi:
Substances chimiques
Aflatoxin B1
9N2N2Y55MH
CYP1A2 protein, human
EC 1.14.14.1
Cytochrome P-450 CYP1A2
EC 1.14.14.1
Phenacetin
ER0CTH01H9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
155-159Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.