Rpd3L and Hda1 histone deacetylases facilitate repair of broken forks by promoting sister chromatid cohesion.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
15 11 2019
15 11 2019
Historique:
received:
13
05
2019
accepted:
22
10
2019
entrez:
16
11
2019
pubmed:
16
11
2019
medline:
11
3
2020
Statut:
epublish
Résumé
Genome stability involves accurate replication and DNA repair. Broken replication forks, such as those encountering a nick, lead to double strand breaks (DSBs), which are preferentially repaired by sister-chromatid recombination (SCR). To decipher the role of chromatin in eukaryotic DSB repair, here we analyze a collection of yeast chromatin-modifying mutants using a previously developed system for the molecular analysis of repair of replication-born DSBs by SCR based on a mini-HO site. We confirm the candidates through FLP-based systems based on a mutated version of the FLP flipase that causes nicks on either the leading or lagging DNA strands. We demonstrate that Rpd3L and Hda1 histone deacetylase (HDAC) complexes contribute to the repair of replication-born DSBs by facilitating cohesin loading, with no effect on other types of homology-dependent repair, thus preventing genome instability. We conclude that histone deacetylation favors general sister chromatid cohesion as a necessary step in SCR.
Identifiants
pubmed: 31729385
doi: 10.1038/s41467-019-13210-5
pii: 10.1038/s41467-019-13210-5
pmc: PMC6858524
doi:
Substances chimiques
Saccharomyces cerevisiae Proteins
0
HDA1 protein, S cerevisiae
EC 3.5.1.-
RPD3 protein, S cerevisiae
EC 3.5.1.-
Histone Deacetylases
EC 3.5.1.98
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5178Références
PLoS Genet. 2013;9(1):e1003237
pubmed: 23357952
EMBO J. 1998 Jan 15;17(2):598-608
pubmed: 9430650
Genes Dev. 1997 Dec 15;11(24):3459-70
pubmed: 9407037
Trends Biochem Sci. 2004 Aug;29(8):389-92
pubmed: 15288867
Mol Cell. 2017 Nov 16;68(4):758-772.e4
pubmed: 29129641
Mol Cell Biol. 2005 Jun;25(12):4903-13
pubmed: 15923609
Nature. 2005 Jul 14;436(7048):294-8
pubmed: 16015338
PLoS Genet. 2017 May 5;13(5):e1006781
pubmed: 28475600
Genetics. 1992 Oct;132(2):387-402
pubmed: 1427035
Cell. 2005 Nov 18;123(4):581-92
pubmed: 16286007
Cell Rep. 2012 Nov 29;2(5):1399-410
pubmed: 23103169
Yeast. 1994 Dec;10(13):1793-808
pubmed: 7747518
Hum Mol Genet. 2008 Jul 15;17(14):2172-80
pubmed: 18411254
Mol Cell. 2012 Oct 12;48(1):98-108
pubmed: 22885006
Genetics. 2019 Feb;211(2):503-513
pubmed: 30559326
Nat Struct Mol Biol. 2013 Mar;20(3):317-25
pubmed: 23377543
Mol Cell. 2004 Dec 22;16(6):1003-15
pubmed: 15610742
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8276-82
pubmed: 11459964
Science. 2015 Aug 14;349(6249):742-7
pubmed: 26273056
Biochim Biophys Acta. 2016 Aug;1866(1):1-11
pubmed: 27207471
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14503-8
pubmed: 8962081
Genes Dev. 2005 Dec 15;19(24):3031-42
pubmed: 16319193
J Biol Chem. 2007 Dec 28;282(52):37805-14
pubmed: 17977840
Mol Cell. 2010 Sep 10;39(5):689-99
pubmed: 20832721
Methods Enzymol. 2006;409:462-76
pubmed: 16793418
Mol Cell. 2010 Sep 10;39(5):677-88
pubmed: 20832720
Yeast. 2004 Aug;21(11):947-62
pubmed: 15334558
Mol Cell. 2016 Jan 7;61(1):15-26
pubmed: 26687679
Genetics. 2002 Oct;162(2):603-14
pubmed: 12399375
Cell. 2017 Feb 9;168(4):644-656
pubmed: 28187286
Cell. 1999 Jul 23;98(2):249-59
pubmed: 10428036
Cold Spring Harb Symp Quant Biol. 2000;65:127-33
pubmed: 12760027
Nat Methods. 2009 Oct;6(10):753-7
pubmed: 19749762
Genetics. 2009 Jun;182(2):437-46
pubmed: 19332880
Nat Rev Cancer. 2015 May;15(5):276-89
pubmed: 25907220
Mol Cell. 2004 Mar 12;13(5):739-50
pubmed: 15023343
EMBO J. 2006 Jul 26;25(14):3377-88
pubmed: 16810316
Cell. 1997 Oct 3;91(1):35-45
pubmed: 9335333
Science. 2007 Jul 13;317(5835):245-8
pubmed: 17626885
Clin Genet. 2009 Oct;76(4):303-14
pubmed: 19793304
Methods Mol Biol. 2011;745:151-72
pubmed: 21660694
Genes Dev. 1998 Mar 15;12(6):797-805
pubmed: 9512514
Exp Cell Res. 2010 May 15;316(9):1445-53
pubmed: 20043905
Hum Genet. 2017 Mar;136(3):307-320
pubmed: 28120103
J Biol Chem. 2002 Nov 22;277(47):45149-53
pubmed: 12228239
Nature. 2002 Aug 29;418(6901):994-8
pubmed: 12198550
Nat Cell Biol. 2002 Jan;4(1):89-93
pubmed: 11780129
Mol Cell. 2010 Mar 12;37(5):690-701
pubmed: 20227372
Mol Cell. 2003 Jun;11(6):1661-71
pubmed: 12820977
Nat Cell Biol. 2006 Sep;8(9):1032-4
pubmed: 16892052
Mol Cancer Ther. 2005 Mar;4(3):361-8
pubmed: 15767545
Curr Biol. 2001 Jun 26;11(12):991-5
pubmed: 11448778
Science. 2001 Dec 21;294(5551):2539-42
pubmed: 11598266
Science. 2017 Mar 24;355(6331):1330-1334
pubmed: 28336671
Mol Cell Biol. 2011 Oct;31(19):3924-37
pubmed: 21807899
Science. 2007 Jul 13;317(5835):242-5
pubmed: 17626884
Nucleic Acids Res. 2007;35(19):6560-70
pubmed: 17905819
Mol Cell Biol. 2004 Feb;24(3):1232-44
pubmed: 14729968
Mol Microbiol. 2010 May;76(4):1049-62
pubmed: 20398213
EMBO J. 2017 Dec 1;36(23):3532-3547
pubmed: 29074626
Mol Cell. 2004 Dec 22;16(6):991-1002
pubmed: 15610741
DNA Repair (Amst). 2015 Aug;32:149-157
pubmed: 25957489
PLoS Genet. 2011 Feb 03;7(2):e1002000
pubmed: 21304892
Cell Mol Life Sci. 2009 Mar;66(6):1039-56
pubmed: 19153654
EMBO J. 2000 Jul 3;19(13):3398-407
pubmed: 10880452
Proc Natl Acad Sci U S A. 1987 Sep;84(17):6215-9
pubmed: 3306671
Mol Cell Biol. 2009 Oct;29(20):5441-54
pubmed: 19651902
Curr Biol. 2007 Apr 3;17(7):630-6
pubmed: 17349791
EMBO Rep. 2006 Sep;7(9):919-26
pubmed: 16888651
Yeast. 1998 Jun 30;14(9):847-52
pubmed: 9818722
Nucleic Acids Res. 2013 Feb 1;41(3):1669-83
pubmed: 23254329
Hum Mol Genet. 2007 Jun 15;16(12):1478-87
pubmed: 17468178
PLoS One. 2010 Aug 12;5(8):e12143
pubmed: 20711347
Curr Biol. 2002 Oct 1;12(19):1652-60
pubmed: 12361567