Native Chromatin Proteomics Reveals a Role for Specific Nucleoporins in Heterochromatin Organization and Maintenance.
Cell Nucleus
/ metabolism
Chromatin
/ metabolism
Chromatin Assembly and Disassembly
/ physiology
DNA, Ribosomal
/ metabolism
Epigenesis, Genetic
/ physiology
Euchromatin
/ metabolism
Heterochromatin
/ metabolism
Nuclear Pore Complex Proteins
/ metabolism
Proteomics
/ methods
Schizosaccharomyces
/ metabolism
Schizosaccharomyces pombe Proteins
/ metabolism
Transcription, Genetic
/ physiology
Bdf1
Clr4
H3K9me
HP1
INM proteins
NPC
SUV39H
Swi6
epigenetic inheritance
nucleoporins
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
02 01 2020
02 01 2020
Historique:
received:
06
03
2019
revised:
19
08
2019
accepted:
11
10
2019
pubmed:
1
12
2019
medline:
28
3
2020
entrez:
1
12
2019
Statut:
ppublish
Résumé
Spatially and functionally distinct domains of heterochromatin and euchromatin play important roles in the maintenance of chromosome stability and regulation of gene expression, but a comprehensive knowledge of their composition is lacking. Here, we develop a strategy for the isolation of native Schizosaccharomyces pombe heterochromatin and euchromatin fragments and analyze their composition by using quantitative mass spectrometry. The shared and euchromatin-specific proteomes contain proteins involved in DNA and chromatin metabolism and in transcription, respectively. The heterochromatin-specific proteome includes all proteins with known roles in heterochromatin formation and, in addition, is enriched for subsets of nucleoporins and inner nuclear membrane (INM) proteins, which associate with different chromatin domains. While the INM proteins are required for the integrity of the nucleolus, containing ribosomal DNA repeats, the nucleoporins are required for aggregation of heterochromatic foci and epigenetic inheritance. The results provide a comprehensive picture of heterochromatin-associated proteins and suggest a role for specific nucleoporins in heterochromatin function.
Identifiants
pubmed: 31784357
pii: S1097-2765(19)30796-8
doi: 10.1016/j.molcel.2019.10.018
pmc: PMC7224636
mid: NIHMS1547202
pii:
doi:
Substances chimiques
Chromatin
0
DNA, Ribosomal
0
Euchromatin
0
Heterochromatin
0
Nuclear Pore Complex Proteins
0
Schizosaccharomyces pombe Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
51-66.e8Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM072805
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA196539
Pays : United States
Organisme : NIDDK NIH HHS
ID : K01 DK098285
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM110174
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM132129
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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