Crystal structures and calorimetry reveal catalytically relevant binding mode of coproporphyrin and coproheme in coproporphyrin ferrochelatase.
Amino Acid Sequence
Bacterial Proteins
/ chemistry
Binding Sites
Calorimetry
Catalysis
Coproporphyrins
/ chemistry
Crystallography, X-Ray
Ferrochelatase
/ chemistry
Heme
/ chemistry
Kinetics
Listeria monocytogenes
/ enzymology
Models, Molecular
Phylogeny
Protein Binding
Protein Conformation
Sequence Homology
X-ray crystallography
enzyme kinetics
ferrochelatase
heme biosynthesis
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
25
10
2019
revised:
18
11
2019
accepted:
02
12
2019
pubmed:
4
12
2019
medline:
11
5
2021
entrez:
4
12
2019
Statut:
ppublish
Résumé
Coproporphyrin ferrochelatases (CpfCs, EC 4.99.1.9) insert ferrous iron into coproporphyrin III yielding coproheme. CpfCs are utilized by prokaryotic, mainly monoderm (Gram-positive) bacteria within the recently detected coproporphyrin-dependent (CPD) heme biosynthesis pathway. Here, we present a comprehensive study on CpfC from Listeria monocytogenes (LmCpfC) including the first crystal structure of a coproheme-bound CpfC. Comparison of crystal structures of apo-LmCpfC and coproheme-LmCpfC allowed identification of structural rearrangements and of amino acids involved in tetrapyrrole macrocycle and Fe
Identifiants
pubmed: 31794133
doi: 10.1111/febs.15164
pmc: PMC7340540
mid: EMS85422
doi:
Substances chimiques
Bacterial Proteins
0
Coproporphyrins
0
Heme
42VZT0U6YR
Ferrochelatase
EC 4.99.1.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2779-2796Subventions
Organisme : Wellcome Trust
ID : 201543
Pays : United Kingdom
Organisme : Austrian Science Fund FWF
ID : P 29099
Pays : Austria
Organisme : Wellcome Trust Collaborative Award
ID : 201543/Z/16
Informations de copyright
© 2019 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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