Restoration of Functional Full-Length Dystrophin After Intramuscular Transplantation of Foamy Virus-Transduced Myoblasts.


Journal

Human gene therapy
ISSN: 1557-7422
Titre abrégé: Hum Gene Ther
Pays: United States
ID NLM: 9008950

Informations de publication

Date de publication:
02 2020
Historique:
pubmed: 6 12 2019
medline: 4 6 2021
entrez: 6 12 2019
Statut: ppublish

Résumé

Stem cell therapy is a promising strategy to treat muscle diseases such as Duchenne muscular dystrophy (DMD). To avoid immune rejection of donor cells or donor-derived muscle, autologous cells, which have been genetically modified to express dystrophin, are preferable to cells derived from healthy donors. Restoration of full-length dystrophin (FL-dys) using viral vectors is extremely challenging, due to the limited packaging capacity of the vectors, but we have recently shown that either a foamy viral or lentiviral vector is able to package FL-dys open-reading frame and transduce myoblasts derived from a DMD patient. Differentiated myotubes derived from these transduced cells produced FL-dys. Here, we transplanted the foamy viral dystrophin-corrected DMD myoblasts intramuscularly into

Identifiants

pubmed: 31801386
doi: 10.1089/hum.2019.224
pmc: PMC7047098
doi:

Substances chimiques

AC133 Antigen 0
Biomarkers 0
Dystrophin 0
Sarcoglycans 0
Nitric Oxide Synthase Type I EC 1.14.13.39

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

241-252

Subventions

Organisme : Medical Research Council
ID : G0900872
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 093610/Z/10/A
Pays : United Kingdom
Organisme : Department of Health
Pays : United Kingdom

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Auteurs

Jinhong Meng (J)

Developmental Neuroscience Programme, Molecular Neurosciences Section, Dubowitz Neuromuscular Centre, UCL Great Ormond Street Institute of Child Health, London, United Kingdom.
National Institute for Health Research, Great Ormond Street Institute of Child Health Biomedical Research Centre, University College London, London, United Kingdom.

Nathan Paul Sweeney (NP)

Jefferiss Research Trust Laboratories, Imperial College London, London, United Kingdom.

Bruno Doreste (B)

Developmental Neuroscience Programme, Molecular Neurosciences Section, Dubowitz Neuromuscular Centre, UCL Great Ormond Street Institute of Child Health, London, United Kingdom.
National Institute for Health Research, Great Ormond Street Institute of Child Health Biomedical Research Centre, University College London, London, United Kingdom.

Francesco Muntoni (F)

Developmental Neuroscience Programme, Molecular Neurosciences Section, Dubowitz Neuromuscular Centre, UCL Great Ormond Street Institute of Child Health, London, United Kingdom.
National Institute for Health Research, Great Ormond Street Institute of Child Health Biomedical Research Centre, University College London, London, United Kingdom.

Myra McClure (M)

Jefferiss Research Trust Laboratories, Imperial College London, London, United Kingdom.

Jennifer Morgan (J)

Developmental Neuroscience Programme, Molecular Neurosciences Section, Dubowitz Neuromuscular Centre, UCL Great Ormond Street Institute of Child Health, London, United Kingdom.
National Institute for Health Research, Great Ormond Street Institute of Child Health Biomedical Research Centre, University College London, London, United Kingdom.

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Classifications MeSH