Efficient production of phenylpropionic acids by an amino-group-transformation biocatalytic cascade.
amino group
biocatalysis
enzyme engineering
multienzyme cascade
phenylpropionic acids
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
26
08
2019
revised:
06
11
2019
accepted:
24
11
2019
pubmed:
6
12
2019
medline:
23
2
2021
entrez:
6
12
2019
Statut:
ppublish
Résumé
Phenylpropionic acids are commonly used in the synthesis of pharmaceuticals, cosmetics, and fine chemicals. However, the synthesis of phenylpropionic acids faces the challenges of high cost of substrates and a limited range of products. Here, we present an artificially designed amino-group-transformation biocatalytic process, which uses simple phenols, pyruvate, and ammonia to synthesize diverse phenylpropionic acids. This biocatalytic cascade comprises an amino-group-introduction module and three amino-group-transformation modules, and operates in a modular assembly manner. Escherichia coli catalysts coexpressing enzymes from different modules achieve whole-cell simultaneous one-pot transformations of phenols into the corresponding phenylpropionic acids including (S)-α-amino acids, α-keto acids, (R)-α-amino acids, and (R)-β-amino acids. With cofactor recycling, protein engineering, and transformation optimization, four (S)-α-amino acids, four α-keto acids, four (R)-α-amino acids, and four (R)-β-amino acids are synthesized with good conversion (68-99%) and high enantioselectivities (>98%). Therefore, the amino-group-transformation concept provides a universal and efficient tool for synthesizing diverse products.
Substances chimiques
Amino Acids
0
Enzymes
0
Phenols
0
Phenylpropionates
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
614-625Subventions
Organisme : National First-Class Discipline Program of Light Industry Technology and Engineering
ID : LITE2018-08
Pays : International
Organisme : Key Technologies R&D Program of Jiangsu Province
ID : BE2018623
Pays : International
Organisme : National Natural Science Foundation of China
ID : 21878126
Pays : International
Informations de copyright
© 2019 Wiley Periodicals, Inc.
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