Mechanical Stimuli Affect Escherichia coli Heat-Stable Enterotoxin-Cyclic GMP Signaling in a Human Enteroid Intestine-Chip Model.
Bacterial Toxins
Cyclic GMP
/ physiology
Enterotoxigenic Escherichia coli
/ physiology
Enterotoxins
/ physiology
Escherichia coli Infections
/ physiopathology
Escherichia coli Proteins
/ physiology
Humans
Intestine, Small
/ physiology
Jejunum
/ cytology
Signal Transduction
/ physiology
Stress, Mechanical
Virulence Factors
/ physiology
ETEC
Intestine-Chip
enteroids
heat-stable enterotoxin
mechanical stress
peristalsis
Journal
Infection and immunity
ISSN: 1098-5522
Titre abrégé: Infect Immun
Pays: United States
ID NLM: 0246127
Informations de publication
Date de publication:
20 02 2020
20 02 2020
Historique:
received:
18
11
2019
accepted:
23
11
2019
pubmed:
11
12
2019
medline:
24
4
2020
entrez:
11
12
2019
Statut:
epublish
Résumé
Modeling host-pathogen interactions with human intestinal epithelia using enteroid monolayers on permeable supports (such as Transwells) represents an alternative to animal studies or use of colon cancer-derived cell lines. However, the static monolayer model does not expose epithelial cells to mechanical forces normally present in the intestine, including luminal flow and serosal blood flow (shear force) or peristaltic forces. To determine the contribution of mechanical forces in the functional response of human small intestine to a virulence factor of a pathogenic intestinal bacterium, human jejunal enteroids were cultured as monolayers in microengineered fluidic-based Organ-Chips (Intestine-Chips) exposed to enterotoxigenic
Identifiants
pubmed: 31818966
pii: IAI.00866-19
doi: 10.1128/IAI.00866-19
pmc: PMC7035931
pii:
doi:
Substances chimiques
Bacterial Toxins
0
Enterotoxins
0
Escherichia coli Proteins
0
Virulence Factors
0
heat stable toxin (E coli)
0
Cyclic GMP
H2D2X058MU
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R01 AI089894
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK089502
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI125181
Pays : United States
Organisme : BLRD VA
ID : I01 BX004825
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK116352
Pays : United States
Informations de copyright
Copyright © 2020 American Society for Microbiology.
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