Nanopore Targeted Sequencing for Rapid Gene Mutations Detection in Acute Myeloid Leukemia.
Adult
Alleles
CCAAT-Enhancer-Binding Proteins
/ genetics
Female
High-Throughput Nucleotide Sequencing
/ methods
Humans
Isocitrate Dehydrogenase
/ genetics
Leukemia, Myeloid, Acute
/ genetics
Male
Mutation
Nanopores
Nuclear Proteins
/ genetics
Nucleophosmin
Prognosis
Sequence Analysis, DNA
/ methods
Tumor Suppressor Protein p53
/ genetics
fms-Like Tyrosine Kinase 3
/ genetics
FLT3 internal tandem duplications
acute myeloid leukemia
biallelic CEBPA mutations
mutational analysis
nanopore targeted sequencing
Journal
Genes
ISSN: 2073-4425
Titre abrégé: Genes (Basel)
Pays: Switzerland
ID NLM: 101551097
Informations de publication
Date de publication:
09 12 2019
09 12 2019
Historique:
received:
12
11
2019
revised:
05
12
2019
accepted:
05
12
2019
entrez:
15
12
2019
pubmed:
15
12
2019
medline:
2
6
2020
Statut:
epublish
Résumé
Acute myeloid leukemia (AML) clinical settings cannot do without molecular testing to confirm or rule out predictive biomarkers for prognostic stratification, in order to initiate or withhold targeted therapy. Next generation sequencing offers the advantage of the simultaneous investigation of numerous genes, but these methods remain expensive and time consuming. In this context, we present a nanopore-based assay for rapid (24 h) sequencing of six genes (
Identifiants
pubmed: 31835432
pii: genes10121026
doi: 10.3390/genes10121026
pmc: PMC6947272
pii:
doi:
Substances chimiques
CCAAT-Enhancer-Binding Proteins
0
CEBPA protein, human
0
NPM1 protein, human
0
Nuclear Proteins
0
TP53 protein, human
0
Tumor Suppressor Protein p53
0
Nucleophosmin
117896-08-9
IDH2 protein, human
EC 1.1.1.41
Isocitrate Dehydrogenase
EC 1.1.1.41
IDH1 protein, human
EC 1.1.1.42.
FLT3 protein, human
EC 2.7.10.1
fms-Like Tyrosine Kinase 3
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Sci Rep. 2018 Aug 7;8(1):11798
pubmed: 30087429
Oncogene. 2010 Apr 29;29(17):2509-16
pubmed: 20101201
J Clin Pathol. 2016 Sep;69(9):801-4
pubmed: 26896490
Leukemia. 2006 Dec;20(12):2190-2
pubmed: 17024113
Blood. 2010 Jan 21;115(3):453-74
pubmed: 19880497
Microb Genom. 2016 Sep 20;2(9):e000087
pubmed: 28785419
J Mol Diagn. 2018 Jan;20(1):4-27
pubmed: 29154853
Blood. 2014 Nov 27;124(23):3441-9
pubmed: 25270908
J Mol Diagn. 2019 Mar;21(2):228-240
pubmed: 30576870
Genomics Proteomics Bioinformatics. 2016 Oct;14(5):265-279
pubmed: 27646134
Blood. 2016 May 19;127(20):2391-405
pubmed: 27069254
Electrophoresis. 2013 Aug;34(15):2137-44
pubmed: 23744714
Brief Bioinform. 2018 Nov 27;19(6):1256-1272
pubmed: 28637243
N Engl J Med. 2018 Jun 21;378(25):2386-2398
pubmed: 29860938
Haematologica. 2019 Jan;104(1):e9-e12
pubmed: 30076182
Diagn Pathol. 2016 Oct 10;11(1):96
pubmed: 27724982
Arch Pathol Lab Med. 2017 Jun;141(6):759-775
pubmed: 28557600
Nat Methods. 2018 Jun;15(6):461-468
pubmed: 29713083
Bioinformatics. 2010 Mar 1;26(5):589-95
pubmed: 20080505
J Mol Diagn. 2017 May;19(3):341-365
pubmed: 28341590
Leuk Res. 2011 Nov;35(11):e220-2
pubmed: 21794915
Blood. 1999 May 1;93(9):3074-80
pubmed: 10216104
Nat Methods. 2015 Apr;12(4):351-6
pubmed: 25686389
Oncotarget. 2018 Jan 5;9(13):10978-10986
pubmed: 29541390
Sci Rep. 2018 Feb 16;8(1):3159
pubmed: 29453452
Hum Pathol. 2018 Oct;80:82-86
pubmed: 29530751
Nat Commun. 2017 Nov 6;8(1):1326
pubmed: 29109544
Blood. 2017 Aug 10;130(6):722-731
pubmed: 28588020
Leukemia. 2016 May;30(5):1220-1225
pubmed: 26487272
J Mol Diagn. 2019 Jan;21(1):89-98
pubmed: 30577887
Nat Biotechnol. 2011 Jan;29(1):24-6
pubmed: 21221095
Blood. 2017 Jan 26;129(4):424-447
pubmed: 27895058
DNA Res. 2017 Dec 1;24(6):585-596
pubmed: 29117310
Leukemia. 2007 Oct;21(10):2221-5
pubmed: 17554380
Genome Res. 1999 Sep;9(9):868-77
pubmed: 10508846
Exp Mol Pathol. 2017 Aug;103(1):33-37
pubmed: 28663031
Blood. 2012 Mar 1;119(9):2114-21
pubmed: 22186996
Oncotarget. 2018 Jul 10;9(53):30128-30145
pubmed: 30046393
J Clin Oncol. 2010 Jun 1;28(16):2739-47
pubmed: 20439648
N Engl J Med. 2017 Aug 3;377(5):454-464
pubmed: 28644114
Sci Rep. 2018 Jul 19;8(1):10931
pubmed: 30026559