Direct observation of damage clustering in irradiated DNA with atomic force microscopy.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
20 02 2020
20 02 2020
Historique:
accepted:
29
11
2019
revised:
23
11
2019
received:
24
05
2019
pubmed:
17
12
2019
medline:
20
3
2020
entrez:
17
12
2019
Statut:
ppublish
Résumé
Ionizing radiation produces clustered DNA damage that contains two or more lesions in 10-20 bp. It is believed that the complexity of clustered damage (i.e., the number of lesions per damage site) is related to the biological severity of ionizing radiation. However, only simple clustered damage containing two vicinal lesions has been demonstrated experimentally. Here we developed a novel method to analyze the complexity of clustered DNA damage. Plasmid DNA was irradiated with densely and sparsely ionizing Fe-ion beams and X-rays, respectively. Then, the resulting DNA lesions were labeled with biotin/streptavidin and observed with atomic force microscopy. Fe-ion beams produced complex clustered damage containing 2-4 lesions. Furthermore, they generated two or three clustered damage sites in a single plasmid molecule that resulted from the hit of a single track of Fe-ion beams. Conversely, X-rays produced relatively simple clustered damage. The present results provide the first experimental evidence for complex cluster damage.
Identifiants
pubmed: 31840169
pii: 5678522
doi: 10.1093/nar/gkz1159
pmc: PMC7026632
doi:
Substances chimiques
DNA
9007-49-2
Iron
E1UOL152H7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e18Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
J Biol Chem. 1997 Aug 1;272(31):19095-8
pubmed: 9235895
Radiat Res. 1996 Feb;145(2):200-9
pubmed: 8606930
Radiat Res. 2002 Jun;157(6):611-6
pubmed: 12005538
Front Oncol. 2016 Jun 10;6:130
pubmed: 27376024
J Radiat Res. 2004 Jun;45(2):229-37
pubmed: 15304965
Proc Natl Acad Sci U S A. 2015 May 19;112(20):E2575-84
pubmed: 25941401
Radiat Res. 2001 Nov;156(5 Pt 2):577-83
pubmed: 11604075
Cancer Res. 2002 Nov 1;62(21):6263-9
pubmed: 12414656
J Biol Chem. 2000 Feb 18;275(7):4956-64
pubmed: 10671534
Radiat Res. 1985 Sep;103(3):383-92
pubmed: 2994167
DNA Repair (Amst). 2010 Dec 10;9(12):1219-28
pubmed: 21035408
Radiat Res. 2009 Sep;172(3):288-95
pubmed: 19708777
Radiat Res. 2008 Jun;169(6):649-59
pubmed: 18494540
Br J Radiol. 2014 Mar;87(1035):20130626
pubmed: 24198199
Radiat Res. 2015 May;183(5):525-40
pubmed: 25909147
Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10569-74
pubmed: 16024726
Int J Radiat Biol. 1994 Jan;65(1):7-17
pubmed: 7905912
Mutat Res. 2011 Jun 3;711(1-2):28-40
pubmed: 21281649
Proc Natl Acad Sci U S A. 2011 May 17;108(20):8293-8
pubmed: 21527720
Proc Natl Acad Sci U S A. 2000 Jan 4;97(1):103-8
pubmed: 10618378
Int J Radiat Biol. 1996 Nov;70(5):493-503
pubmed: 8947529
Int J Radiat Biol. 1994 Nov;66(5):427-32
pubmed: 7983426
DNA Repair (Amst). 2011 Apr 3;10(4):427-37
pubmed: 21342792
Mutat Res. 2003 Oct 29;531(1-2):93-107
pubmed: 14637248
Front Oncol. 2016 Feb 12;6:23
pubmed: 26904502
Radiat Res. 2002 May;157(5):589-95
pubmed: 11966325
Free Radic Biol Med. 2017 Jun;107:136-145
pubmed: 27894771
Biochemistry. 1993 Nov 16;32(45):12105-11
pubmed: 8218289
Radiat Res. 2007 Jul;168(1):98-105
pubmed: 17722995
Radiat Res. 1996 Feb;145(2):188-99
pubmed: 8606929
Front Mol Biosci. 2015 Jan 29;2:1
pubmed: 25988169
Biochemistry. 2003 Mar 25;42(11):3375-84
pubmed: 12641470
Free Radic Biol Med. 2017 Jun;107:125-135
pubmed: 27939934
Free Radic Res. 2012 Apr;46(4):382-419
pubmed: 22276778
Nucleic Acids Res. 2001 Mar 15;29(6):1285-92
pubmed: 11238994
Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7426-30
pubmed: 11404468
Nucleic Acids Res. 1999 Oct 15;27(20):4001-7
pubmed: 10497264
Mutat Res. 2011 Jun 3;711(1-2):134-41
pubmed: 21130102
Nucleic Acids Res. 2002 Aug 1;30(15):3464-72
pubmed: 12140332
J Biol Chem. 2004 Apr 2;279(14):14464-71
pubmed: 14734554
Carcinogenesis. 1999 May;20(5):905-9
pubmed: 10334210
Radiat Res. 2006 Nov;166(5):767-81
pubmed: 17067210
Nucleic Acids Res. 2001 Jan 15;29(2):430-8
pubmed: 11139613
Biochemistry. 1993 Aug 17;32(32):8276-83
pubmed: 8347625
DNA Repair (Amst). 2015 Apr;28:93-106
pubmed: 25659339
Sci Rep. 2017 Jan 17;7:40616
pubmed: 28094292