The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast.
DNA replication
Rad52
S. pombe
chromosomes
gene expression
homologous recombination
replication fork barrier
replication fork collapse
replication termination
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
19 12 2019
19 12 2019
Historique:
received:
29
06
2019
accepted:
18
12
2019
pubmed:
20
12
2019
medline:
23
5
2020
entrez:
20
12
2019
Statut:
epublish
Résumé
Previously we reported that a process called inter-fork strand annealing (IFSA) causes genomic deletions during the termination of DNA replication when an active replication fork converges on a collapsed fork (Morrow et al., 2017). We also identified the FANCM-related DNA helicase Fml1 as a potential suppressor of IFSA. Here, we confirm that Fml1 does indeed suppress IFSA, and show that this function depends on its catalytic activity and ability to interact with Mhf1-Mhf2 via its C-terminal domain. Finally, a plausible mechanism of IFSA suppression is demonstrated by the finding that Fml1 can catalyse regressed fork restoration in vitro.
Identifiants
pubmed: 31855181
doi: 10.7554/eLife.49784
pii: 49784
pmc: PMC6952179
doi:
pii:
Substances chimiques
Chromosomal Proteins, Non-Histone
0
Mhf2 protein, S pombe
0
Schizosaccharomyces pombe Proteins
0
Fml1 protein, S pombe
EC 3.6.1.-
DNA Helicases
EC 3.6.4.-
Mhf1 protein, S pombe
EC 3.6.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P019706/1
Pays : United Kingdom
Organisme : Wellcome
ID : 090767/Z/09/Z
Pays : International
Informations de copyright
© 2019, Wong et al.
Déclaration de conflit d'intérêts
IW, JN, JO, SS, FO, SC, MW No competing interests declared
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