Development of glutaric acid production consortium system with α-ketoglutaric acid regeneration by glutamate oxidase in Escherichia coli.

Amino Acid Oxidoreductases / metabolism Catalase / metabolism Escherichia coli / enzymology Fermentation Glutarates / metabolism Ketoglutaric Acids / metabolism Metabolic Engineering / methods
Catalase Glutamate oxidase Glutaric acid Optimization α-Ketoglutaric acid

Journal

Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761

Informations de publication

Date de publication:
Feb 2020
Historique:
received: 08 08 2019
revised: 10 09 2019
accepted: 07 10 2019
entrez: 26 12 2019
pubmed: 26 12 2019
medline: 1 5 2020
Statut: ppublish

Résumé

Glutaric acid is a C5 dicarboxylic acid that can be used as a building block for bioplastics. Although high concentrations of glutaric acid can be produced by fermentation or bioconversion, a large amount of α-ketoglutaric acid (α-KG) is necessary to accept the amine group from 5-aminovaleric acid. To decrease the demand for α-KG, we introduced l-glutamate oxidase (GOX) from Streptomyces mobaraensis in our previous system for cofactor regeneration in combination with a glutaric acid production system from 5-aminovaleric acid. To enhance glutaric acid production, critical factors were optimized such as the expression vector, pH, temperature, and cell ratio. As a result, the demand for α-KG was decreased by more than 6-fold under optimized conditions. Additionally, the effect of catalase was also demonstrated by blocking the degradation of α-KG to succinic acid because of the hydrogen peroxide. Finally, 468.5 mM glutaric acid was produced from 800 mM 5-aminovaleric acid using only 120 mM α-KG. Moreover, this system containing davBA, gabTD-nox, and gox can be applied to produce glutaric acid from L-lysine by reusing α-KG with GOX. This improved cofactor regeneration system has a potential to apply much larger production of glutaric acid.

Identifiants

DOI: 10.1016/j.enzmictec.2019.109446 PMID: 31874692
pubmed: 31874692
pii: S0141-0229(19)30184-X
doi: 10.1016/j.enzmictec.2019.109446
pii:
doi:

Substances chimiques

Glutarates 0
Ketoglutaric Acids 0
Catalase EC 1.11.1.6
Amino Acid Oxidoreductases EC 1.4.-
L-glutamate oxidase EC 1.4.3.11
glutaric acid H849F7N00B

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109446

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Soo-Yeon Yang (SY)

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, South Korea.

Tae-Rim Choi (TR)

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, South Korea.

Hye-Rim Jung (HR)

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, South Korea.

Ye-Lim Park (YL)

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, South Korea.

Yeong-Hoon Han (YH)

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, South Korea.

Hun-Suk Song (HS)

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, South Korea.

Ranjit Gurav (R)

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, South Korea.

Shashi Kant Bhatia (SK)

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, South Korea; Institute for Ubiquitous Information Technology and Applications, Konkuk University, Seoul, 05029, South Korea.

Kyungmoon Park (K)

Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong City, Republic of Korea.

Jung-Oh Ahn (JO)

Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), Gwahangno, Yuseong-Gu, Daejeon, 305-806, Republic of Korea.

Yung-Hun Yang (YH)

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, South Korea; Institute for Ubiquitous Information Technology and Applications, Konkuk University, Seoul, 05029, South Korea. Electronic address: seokor@konkuk.ac.kr.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxidoreductases acting on CH-NH2 group donors › Amino-acid oxidoreductases › Development of glutaric acid production...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Peroxidases › Catalase › Development of glutaric acid production...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Development of glutaric acid production...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Fermentation › Development of glutaric acid production...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Diacides carboxyliques › Glutarates › Development of glutaric acid production...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Cétoacides › Acides cétoglutariques › Development of glutaric acid production...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Génie métabolique › Development of glutaric acid production...