Mutational Analysis of N-Ethyl-N-Nitrosourea (ENU) in the Fission Yeast
ATIC
Ade10
N-ethyl-N-nitrosourea
S.pombe
auxotrophy
mutagenesis
phosphotransferase
ribosiduria
Journal
G3 (Bethesda, Md.)
ISSN: 2160-1836
Titre abrégé: G3 (Bethesda)
Pays: England
ID NLM: 101566598
Informations de publication
Date de publication:
05 03 2020
05 03 2020
Historique:
pubmed:
5
1
2020
medline:
13
4
2021
entrez:
5
1
2020
Statut:
epublish
Résumé
Forward genetics in model organisms has boosted our knowledge of the genetic bases of development, aging, and human diseases. In this experimental pipeline, it is crucial to start by inducing a large number of random mutations in the genome of the model organism to search for phenotypes of interest. Many chemical mutagens are used to this end because most of them display particular reactivity properties and act differently over DNA. Here we report the use of N-ethyl-N-nitrosourea (ENU) as a mutagen in the fission yeast
Identifiants
pubmed: 31900332
pii: g3.119.400936
doi: 10.1534/g3.119.400936
pmc: PMC7056981
doi:
Substances chimiques
Mutagens
0
Ethyl Methanesulfonate
9H154DI0UP
Ethylnitrosourea
P8M1T4190R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
917-923Informations de copyright
Copyright © 2020 Hoyos-Manchado et al.
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