Isobutanol production in Corynebacterium glutamicum: Suppressed succinate by-production by pckA inactivation and enhanced productivity via the Entner-Doudoroff pathway.

Bacterial Proteins / genetics Butanols / metabolism Corynebacterium glutamicum / genetics Metabolic Engineering Phosphoenolpyruvate Carboxylase / genetics Succinic Acid / metabolism
Corynebacterium glutamicum Isobutanol Phosphoenolpyruvate carboxykinase Redox balance The Entner-Doudoroff pathway

Journal

Metabolic engineering
ISSN: 1096-7184
Titre abrégé: Metab Eng
Pays: Belgium
ID NLM: 9815657

Informations de publication

Date de publication:
05 2020
Historique:
received: 23 08 2019
revised: 24 12 2019
accepted: 07 01 2020
pubmed: 12 1 2020
medline: 28 1 2021
entrez: 12 1 2020
Statut: ppublish

Résumé

On the basis of our previous studies of microbial L-valine production under oxygen deprivation, we developed isobutanol-producing Corynebacterium glutamicum strains. The artificial isobutanol synthesis pathway was composed of the first three steps of the L-valine synthesis pathway; and the subsequent Ehrlich Pathway: pyruvate was converted to 2-ketoisovalerate in the former reactions; and the 2-keto acid was decarboxylated into isobutyraldehyde, and subsequently reduced into isobutanol in the latter reactions. Although there exists redox cofactor imbalance in the overall reactions, i.e., NADH is generated via glycolysis whereas NADPH is required to synthesize isobutanol, it was resolved by taking advantage of the NAD-preferring mutant acetohydroxy acid isomeroreductase encoded by ilvC

Identifiants

DOI: 10.1016/j.ymben.2020.01.004 PMID: 31926306
pubmed: 31926306
pii: S1096-7176(19)30345-3
doi: 10.1016/j.ymben.2020.01.004
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Butanols 0
isobutyl alcohol 56F9Z98TEM
Succinic Acid AB6MNQ6J6L
Phosphoenolpyruvate Carboxylase EC 4.1.1.31

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

24-35

Informations de copyright

Copyright © 2020 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

Auteurs

Satoshi Hasegawa (S)

Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan.

Toru Jojima (T)

Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan.

Masako Suda (M)

Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan.

Masayuki Inui (M)

Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan; Division of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara, 630-0101, Japan. Electronic address: mmg-lab@rite.or.jp.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Isobutanol production in Corynebacterium...
questionsmedicales.fr › Lipides › Alcools gras › Butanols › Isobutanol production in Corynebacterium...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes irréguliers à Gram positif › Corynebacterium › Corynebacterium glutamicum › Isobutanol production in Corynebacterium...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Génie métabolique › Isobutanol production in Corynebacterium...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-carbon lyases › Carboxy-lyases › Phosphoenolpyruvate carboxylase › Isobutanol production in Corynebacterium...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Diacides carboxyliques › Succinates › Acide succinique › Isobutanol production in Corynebacterium...