Association of Perforin and Granzyme-B Levels with Hyperandrogenism in Polycystic Ovary Syndrome: A Case-Control Study.
Adolescent
Adult
Androgens
/ metabolism
Cardiovascular Diseases
/ metabolism
Case-Control Studies
Cytokines
/ metabolism
Dehydroepiandrosterone Sulfate
/ blood
Female
Granzymes
/ blood
Humans
Hyperandrogenism
/ blood
Insulin Resistance
Iran
/ epidemiology
Luteinizing Hormone
/ blood
Perforin
/ blood
Polycystic Ovary Syndrome
/ blood
Risk Factors
Sex Hormone-Binding Globulin
/ analysis
T-Lymphocytes, Cytotoxic
/ metabolism
Testosterone
/ blood
Young Adult
Perforin
androgen
cardiovascular disease
granzyme-B
hyperandrogenemia
polycystic ovary syndrome
sex hormone
sex hormone binding globulin
Journal
Pakistan journal of biological sciences : PJBS
ISSN: 1812-5735
Titre abrégé: Pak J Biol Sci
Pays: Pakistan
ID NLM: 101247723
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
entrez:
14
1
2020
pubmed:
14
1
2020
medline:
28
7
2020
Statut:
ppublish
Résumé
Polycystic ovary syndrome (PCOS) is shown to be associated with hyperandrogenemia and has some features such as cytotoxic T-cells activation and release of perforin and granzyme-B. Present work was aimed to investigate the relation of perforin and granzyme-B to androgenic state in PCOS. Forty three PCOS and 40 control women were recruited. After recording demographic data, sex hormone status and cardiovascular disease (CVD) risk factors were evaluated. Perforin and granzyme-B levels were measured using sandwich ELISA kits. Sex hormone binding globulin (SHBG) levels were lower in patients. Luteinizing hormone (LH), free testosterone (FT), dehydroepiandrosterone sulfate (DHEA-S), free androgen index (FAI), perforin and granzyme-B values were higher in PCOS group. Perforin and granzyme-B were positively correlated with FT and FAI and with each other in PCOS group. In patients, granzyme-B and perforin were related with FT and FAI, respectively. The results of present study together with evidences about the release of pro-inflammatory cytokines in insulin resistance, CVD and PCOS suggest that perforin/granzyme-B may be involved in interactions of sex hormones system in PCOS patients.
Sections du résumé
BACKGROUND AND OBJECTIVE
OBJECTIVE
Polycystic ovary syndrome (PCOS) is shown to be associated with hyperandrogenemia and has some features such as cytotoxic T-cells activation and release of perforin and granzyme-B. Present work was aimed to investigate the relation of perforin and granzyme-B to androgenic state in PCOS.
MATERIALS AND METHODS
METHODS
Forty three PCOS and 40 control women were recruited. After recording demographic data, sex hormone status and cardiovascular disease (CVD) risk factors were evaluated. Perforin and granzyme-B levels were measured using sandwich ELISA kits.
RESULTS
RESULTS
Sex hormone binding globulin (SHBG) levels were lower in patients. Luteinizing hormone (LH), free testosterone (FT), dehydroepiandrosterone sulfate (DHEA-S), free androgen index (FAI), perforin and granzyme-B values were higher in PCOS group. Perforin and granzyme-B were positively correlated with FT and FAI and with each other in PCOS group. In patients, granzyme-B and perforin were related with FT and FAI, respectively.
CONCLUSION
CONCLUSIONS
The results of present study together with evidences about the release of pro-inflammatory cytokines in insulin resistance, CVD and PCOS suggest that perforin/granzyme-B may be involved in interactions of sex hormones system in PCOS patients.
Identifiants
pubmed: 31930827
doi: 10.3923/pjbs.2019.393.399
doi:
Substances chimiques
Androgens
0
Cytokines
0
PRF1 protein, human
0
Sex Hormone-Binding Globulin
0
Perforin
126465-35-8
Testosterone
3XMK78S47O
Dehydroepiandrosterone Sulfate
57B09Q7FJR
Luteinizing Hormone
9002-67-9
GZMB protein, human
EC 3.4.21.-
Granzymes
EC 3.4.21.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM