Broomrape infestation in carrot (Daucus carota): Changes in carotenoid gene expression and carotenoid accumulation in the parasitic weed Phelipanche aegyptiaca and its host.
Carotenoids
/ analysis
Chromatography, High Pressure Liquid
Daucus carota
/ growth & development
Dioxygenases
/ genetics
Gene Expression Regulation, Plant
Geranylgeranyl-Diphosphate Geranylgeranyltransferase
/ genetics
Orobanchaceae
/ growth & development
Plant Proteins
/ genetics
Plant Roots
/ metabolism
Plant Weeds
/ growth & development
cis-trans-Isomerases
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 01 2020
15 01 2020
Historique:
received:
10
10
2019
accepted:
26
12
2019
entrez:
17
1
2020
pubmed:
17
1
2020
medline:
11
11
2020
Statut:
epublish
Résumé
Carotenogenesis has been intensively studied in carrot roots, and transcriptional regulation is thought to be the major factor in carotenoid accumulation in these organs. However, little is known about the transcriptional regulation of carotenoid biosynthetic genes concerning carotenoid accumulation during infestation by the obligate parasite Phelipanche aegyptiaca. HPLC analysis revealed a decrease in carotenoid levels of the different carrot cultivars when parasitized by P. aegyptiaca. Besides, we isolated and analyzed P. aegyptiaca tubercles parasitizing the various carrot root cultivars and show that they accumulate different carotenoids compared to those in non-infested carrot roots. Expression analysis of PHYTOENE SYNTHASE (PSY1) and CAROTENOID ISOMERASE (CRTISO) as well as the strigolactone apocarotenoid biosynthetic genes DWARF27 (D27), CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7) and CCD8 revealed that their transcript levels showed significant variation in P. aegyptiaca infested carrot roots. After parasite infestation, the expression of these genes was strongly reduced, as were the carotenoid levels and this was more pronounced in the uncommon non-orange varieties. We also analyzed the parasite genes encoding D27, CCD7 and CCD8 and show that they are expressed in tubercles. This raises important questions of whether the parasite produces its carotenoids and apocarotenoids including strigolactones and whether the latter might have a role in tubercle development.
Identifiants
pubmed: 31942014
doi: 10.1038/s41598-019-57298-7
pii: 10.1038/s41598-019-57298-7
pmc: PMC6962276
doi:
Substances chimiques
Plant Proteins
0
Carotenoids
36-88-4
Dioxygenases
EC 1.13.11.-
Geranylgeranyl-Diphosphate Geranylgeranyltransferase
EC 2.5.1.32
cis-trans-Isomerases
EC 5.2.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
324Références
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